Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.
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- Journal of nanobiotechnology 21:4312023Fe-doped carbon dots: a novel biocompatible nanoplatform for multi-level cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxi Yang,Haiqiu Li,Xinchen Liu,Lei Huang,Boya Zhang,Kexuan Liu,Wangni Xie,Jing Cui,Daowei Li,Laijin Lu,Hongchen Sun,Bai YangPubMed 37978538
- Cells 12:2023Myocardial Mitochondrial DNA Drives Macrophage Inflammatory Response through STING Signaling in Coxsackievirus B3-Induced Viral Myocarditis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndong Qin,Zhenke Wen,Sidong XiongPubMed 37947632
- International journal of general medicine 15:7383-73932022The Clinical Significance of Deglycosylated PD-L1 Level Detection Using 28-8 Monoclonal Antibody in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiyu Wang,Dingyi Gu,Datian Chen,Jie Mei,Xuejing Yang,Junli Ding,Junying Xu,Meilin Wang,Chaoying Liu,Dong HuaPubMed 36164284
- International journal of molecular sciences 23:2022Nebulized Non-Immunogenic Staphylokinase in the Mice Acute Lung Injury Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergey S Markin,Roman D Lapshin,Olga S Baskina,Svetlana A Korotchenko,Irina V Mukhina,Sergei V Ivanov,Mikhail P Semenov,Valerii V Beregovykh,Andrey M SemenovPubMed 36012579
- ACS nano 16:12695-127102022Lipid Nanoparticle Delivery of Fas Plasmid Restores Fas Expression to Suppress Melanoma Growth .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeinab Y Al Subeh,Dakota B Poschel,Priscilla S Redd,John D Klement,Alyssa D Merting,Dafeng Yang,Megh Mehta,Huidong Shi,Yolonda L Colson,Nicholas H Oberlies,Cedric J Pearce,Aaron H Colby,Mark W Grinstaff,Kebin LiuPubMed 35939651
- Quantitative imaging in medicine and surgery 12:3300-33132022Immuno-PET imaging of PD-L1 expression in patient-derived lung cancer xenografts with [Ga]Ga-NOTA-Nb109.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingzhu Liu,Xiaodan Wang,Yanling Yang,Chao Wang,Jian Zou,Jianguo Lin,Ling QiuPubMed 35655844
- International journal of molecular sciences 23:2022Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and Regeneration in Acute Lung Injury Model in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVasily A Kudinov,Rafael I Artyushev,Irina M Zurina,Elena S Zorina,Roman D Lapshin,Ludmila B Snopova,Irina V Mukhina,Irina N SaburinaPubMed 35408778
- Nephron 146:528-5382022Losartan Attenuates Atherosclerosis in Uremic Mice by Regulating Treg/Th17 Balance via Mediating PTEN/PI3K/Akt Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanzhai Wei,Jie Sun,Yibei Li,Kangchun Xu,Man Wang,Yilai ZhangPubMed 35176745
- mBio 12:e01608212021Signaling through Syk or CARD9 Mediates Species-Specific Anti- Protection in Bone Marrow Chimeric Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik Zajta,Katalin Csonka,Adél Tóth,Laszló Tiszlavicz,Tamás Németh,Anita Orosz,Ádám Novák,Máté Csikós,Csaba Vágvölgyi,Attila Mócsai,Attila GácserPubMed 34465030
- Cancer communications (London, England) 41:851-8662021Nuclear Aurora kinase A triggers programmed death-ligand 1-mediated immune suppression by activating MYC transcription in triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShulan Sun,Wei Zhou,Xiaoxi Li,Fei Peng,Min Yan,Yajing Zhan,Fan An,Xiaoyan Li,Yunyong Liu,Quentin Liu,Haozhe PiaoPubMed 34251762
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| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC | Reactivity Reacts | Dilution info - | Notes - |
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Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.
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Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy. Signal amplification is achieved using an indirect polymer detection system. As Rabbit specific IHC polymer detection kit HRP/DAB does not use avidin or biotin, nonspecific staining from endogenous avidin-biotin activity is eliminated and there is no need for additional steps to block endogenous biotin. The primary antibody specific to an antigen on the tissue section is detected by primary antibody Amplifier. The HRP polymer is added to enable detection with signal amplification. Finally, antibody-bound antigen sites are visualized by adding an appropriate substrate/chromogen (not provided).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling USP22 with Anti-USP22 antibody [EPR18945] ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebrum. The section was incubated with Anti-USP22 antibody [EPR18945] ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling USP22 with Anti-USP22 antibody [EPR18945] ab195289 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebrum. The section was incubated with Anti-USP22 antibody [EPR18945] ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling USP22 with Anti-USP22 antibody [EPR18945] ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human breast carcinoma. The section was incubated with Anti-USP22 antibody [EPR18945] ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling USP22 with Anti-USP22 antibody [EPR18945] ab195289 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human cerebrum. The section was incubated with Anti-USP22 antibody [EPR18945] ab195289 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling HSF1 with Anti-HSF1 antibody [EP1710Y] - ChIP Grade ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon. The section was incubated with Anti-HSF1 antibody [EP1710Y] - ChIP Grade ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling HSF1 with Anti-HSF1 antibody [EP1710Y] - ChIP Grade ab52757 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis. The section was incubated with Anti-HSF1 antibody [EP1710Y] - ChIP Grade ab52757 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRP alpha with Anti-SIRP alpha antibody [EPR22930-163] ab260039 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human cerebrum (PMID: 9062191) is observed. The section was incubated with Anti-SIRP alpha antibody [EPR22930-163] ab260039 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemistry of human melanoma tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (Anti-PD-L1 antibody [28-8] ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemistry of human tonsil tissue (formalin/PFA-fixed paraffin embedded sections) stained with Anti-PD-L1 antibody [28-8] (Anti-PD-L1 antibody [28-8] ab205921) and detected with Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Formalin-fixed, paraffin-embedded human heart tissue stained for Alpha B Crystallin using Anti-Alpha B Crystallin antibody [SP288] ab226839 at 1/100 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Positive staining on the human cardiomyocytes, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with Anti-Alpha B Crystallin antibody [SP288] ab226839 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded (A) Neuro-2a (mouse neuroblastoma neuroblast) cell pellet. (B) RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellet. tissue labeling ALK with Anti-ALK antibody [EPR29054-38] ab323643 at 1/100 (5.26 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on (A) Neuro-2a cell pellet, no staining on (B) RAW 264.7 cell pellet.
The section was incubated with Anti-ALK antibody [EPR29054-38] ab323643 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Tissue Microarrays stained for Anti-PD-L1 antibody [28-8] using Anti-PD-L1 antibody [28-8] ab205921 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent). The sections were incubated with Anti-PD-L1 antibody [28-8] ab205921 at +4°C overnight. For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded (A) KARPAS-299 (human T cell lymphoma cell) cell pellet. (B) U-937 (human histiocytic lymphoma monocyte) cell pellet. tissue labeling ALK with Anti-ALK antibody [EPR29054-38] ab323643 at 1/200 (2.63 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on (A) KARPAS-299 cell pellet, no staining on (B) U-937 cell pellet.
The section was incubated with Anti-ALK antibody [EPR29054-38] ab323643 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling hSET1/SET1 with Anti-hSET1/SET1 antibody [EPR26821-25] ab307158 at 1/500 (0.996 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (ab209101). Nuclear staining in mouse testis. The section was incubated with Anti-hSET1/SET1 antibody [EPR26821-25] ab307158 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Alpha B Crystallin using Anti-Alpha B Crystallin antibody [SP288] ab226839 at 1/100 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Positive staining on the tubules in human kidney, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with Anti-Alpha B Crystallin antibody [SP288] ab226839 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CHD5 with Anti-CHD5 antibody [EPR23858-3-8] ab300437 at 1/2000 dilution (0.237 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human spleen. The section was incubated with Anti-CHD5 antibody [EPR23858-3-8] ab300437 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded human cerebrum labeling Pyruvate Dehydrogenase E2 with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human cerebrum.
The section was incubated with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded mouse kidney labeling Pyruvate Dehydrogenase E2 with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on mouse kidney.
The section was incubated with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded human cervical cancer labeling Pyruvate Dehydrogenase E2 with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on human cervical cancer.
The section was incubated with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ING4 with Anti-ING4 antibody [EPR27290-285] ab323509 at 1/50 (10.26 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining in human testis.
The section was incubated with Anti-ING4 antibody [EPR27290-285] ab323509 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labeling ING4 with Anti-ING4 antibody [EPR27290-285] ab323509 at 1/50 (10.26 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining in human cervical squamous carcinoma.
The section was incubated with Anti-ING4 antibody [EPR27290-285] ab323509 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ING4 with Anti-ING4 antibody [EPR27290-285] ab323509 at 1/50 (10.26 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining in human cerebrum.
The section was incubated with Anti-ING4 antibody [EPR27290-285] ab323509 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ING4 with Anti-ING4 antibody [EPR27290-285] ab323509 at 1/50 (10.26 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Low expression: No staining in human skeletal muscle.
The section was incubated with Anti-ING4 antibody [EPR27290-285] ab323509 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PDGFR alpha with Anti-PDGFR alpha antibody [EPR22059-270] ab203491 at 0.26 µg/mL followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human endometrium was observed. The section was incubated with Anti-PDGFR alpha antibody [EPR22059-270] ab203491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on rat colon.The section was incubated with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on mouse colon. The section was incubated with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rabbit IgG, multiclonal [RM1060] - Isotype control with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 at 1/100 (4.91 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on human colon.The section was incubated with Rabbit IgG, multiclonal [RM1060] - Isotype control ab313801 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Formalin-fixed, paraffin-embedded Mouse heart tissue stained for Alpha B Crystallin using Anti-Alpha B Crystallin antibody [SP288] ab226839 at 1/100 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Positive staining on the mouse cardiomyocytes, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with Anti-Alpha B Crystallin antibody [SP288] ab226839 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded rat kidney labeling Pyruvate Dehydrogenase E2 with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on rat kidney.
The section was incubated with Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IKKi/IKKe+NAK/TBK1 with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 at 1/500 (1.088 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human pancreas (PMID: 28069799). The section was incubated with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling IKKi/IKKe+NAK/TBK1 with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 at 1/500 (1.088 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human glioma (PMID: 21171089). The section was incubated with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded mouse lung cancer tissue labeling Smad2 (phospho S250) with Anti-Smad2 (phospho S250) antibody [EPR2855(2)] ab184557 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on mouse lung cancer without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Smad2 (phospho S250) antibody [EPR2855(2)] ab184557 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Panel A. Human colon tissue labeling BCKDHA (phospho S293) with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 at 1/500 (0.948 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mitochondrial staining on human colon without alkaline phosphatase treatment (Panel A). No signal was detected when tissues were treated with alkaline phosphatase (Panel B). The section was incubated with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Panel A. Human colon tissue labeling BCKDHA (phospho S293) with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 at 1/500 (0.948 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mitochondrial staining on human colon cancer without alkaline phosphatase treatment (Panel A). No signal was detected when tissues were treated with alkaline phosphatase (Panel B) (PMID: 32238881). The section was incubated with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Panel A. Mouse kidne tissue labeling BCKDHA (phospho S293) with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 at 1/500 (0.948 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Mitochondrial staining on mouse kidney without alkaline phosphatase treatment (Panel A). No signal was detected when tissues were treated with alkaline phosphatase (Panel B). The section was incubated with Anti-BCKDHA (phospho S293) antibody [EPR27489-76] ab302504 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling IKKi/IKKe+NAK/TBK1 with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 at 1/500 (1.088 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis. The section was incubated with Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] ab289973 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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