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Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals.

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Detection method

Colorimetric

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Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Images

What's included?

100 Test
Components
Empty Mixing Bottle
1 x 1 Unit
Linker 1
1 x 10 mL
Linker 2
1 x 10 mL
Stable DAB/Plus Buffer
1 x 15 mL
Stable DAB/Plus Chromogen
1 x 1 mL
Tissue Primer
1 x 10 mL
Tracer
1 x 10 mL

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Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals.

Key facts

Detection method

Colorimetric

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Rapid DAB Detection System (ab290116) is a powerful Intensification system that significantly enhances chromogenic signals. This system can be integrated into standard immunohistochemistry (IHC) staining methods.


Rapid DAB Detection System is an enzyme-mediated reaction that utilizes horseradish peroxidase (HRP) to catalyze the deposition of two separate Linkers applied sequentially onto tissue sections or cell preparation. The deposited Linkers can be detected with a streptavidin-peroxidase conjugate followed by a reaction with a peroxidase substrate/chromogen solution such as diaminobenzidine (DAB).


This system results in a significant increase in sensitivity compared to standard IHC detection methods, while maintaining similar specificity

Benefits:


  • Rapid three-step immunohistochemistry protocol

  • Time to results in less 30 minutes

  • Significant increase in staining intensity

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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