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StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection.

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What's included?

30 mL
Components
Empty Mixing Dropping Bottle
1 x 1 Unit
StayRed/AP Chromogen
1 x 1 mL
StayRed/AP Substrate Buffer
1 x 30 mL

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StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.

Intended Use:

Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.

Working Solution:

Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.

Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.

Procedure:

  • After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
  • Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
  • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
  • Counterstain sections in hematoxylin.
  • Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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