StayRed/AP 30ml (Alcohol & Xylene Compatible)

Product details

StayRed/AP (ab103741) is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.

Intended Use:

Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.

Working Solution:

Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20 μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.

Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.

Procedure:

• After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
• Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
• Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
• Counterstain sections in hematoxylin.
• Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.

Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Intended Use:

Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.

Working Solution:

Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20 μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.

Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.

Procedure:

• After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
• Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
• Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
• Counterstain sections in hematoxylin.
• Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.