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AB183286

TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green)

5

(1 Review)

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(6 Publications)

Abcam's TripleStain IHC kit (ab183286) is designed to use with user supplied two mouse primary antibodies and one rabbit primary antibody to detect three distinct antigens on a single human tissue or cell samples.
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Immunohistochemistry paraffin embedded sections - TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green) (AB183286)
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Supplier Data

Immunohistochemistry paraffin embedded sections - TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green) (AB183286)

Immunohistochemistry (Paraffin-embedded sections).

Immunohistochemical analysis of paraffin embedded human colon cancer tissue. Mouse anti-smooth muscle actin (red), rabbit anti-CEA (brown), mouse anti-PCNA (emerald).

Key facts

Assay type

Polymer Multiplex

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TripleStain IHC kit (ab183286) is designed to use with user supplied two mouse primary antibodies and one rabbit primary antibody to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue only; however it may also be used on frozen or freshly prepared monolayer cell smears.

Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. This kit supplies polymer enzyme conjugates: Rabbit HRP Polymer, Mouse HRP Polymer and Mouse AP Polymer with three chromogens, DAB Chromogen (brown); Permanent Red Chromogen (red); and Emerald Chromogen (green). Abcam's TripleStain IHC kit (ab183286) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 146:263-282 PubMed37243699

2023

Central and peripheral myeloid-derived suppressor cell-like cells are closely related to the clinical severity of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

María Cristina Ortega,Rafael Lebrón-Galán,Isabel Machín-Díaz,Michelle Naughton,Inmaculada Pérez-Molina,Jennifer García-Arocha,Jose Manuel Garcia-Dominguez,Haydee Goicoechea-Briceño,Virginia Vila-Del Sol,Víctor Quintanero-Casero,Rosa García-Montero,Victoria Galán,Leticia Calahorra,Celia Camacho-Toledano,María Luisa Martínez-Ginés,Denise C Fitzgerald,Diego Clemente

Emerging infectious diseases 28:510-517 PubMed35138244

2022

Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Reagan-Steiner,Julu Bhatnagar,Roosecelis B Martines,Nicholas S Milligan,Carly Gisondo,Frank B Williams,Elizabeth Lee,Lindsey Estetter,Hannah Bullock,Cynthia S Goldsmith,Pamela Fair,Julie Hand,Gillian Richardson,Kate R Woodworth,Titilope Oduyebo,Romeo R Galang,Rebecca Phillips,Elizaveta Belyaeva,Xiao-Ming Yin,Dana Meaney-Delman,Timothy M Uyeki,Drucilla J Roberts,Sherif R Zaki

The American journal of pathology 191:2147-2171 PubMed34428422

2021

Label-Free Quantitative Phase Imaging Reveals Spatial Heterogeneity of Extracellular Vesicles in Select Colon Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Łukasz Zadka,Igor Buzalewicz,Agnieszka Ulatowska-Jarża,Agnieszka Rusak,Maria Kochel,Ireneusz Ceremuga,Piotr Dzięgiel

Cell reports 35:109220 PubMed34107253

2021

Bacterial genotoxins induce T cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah L Mathiasen,Laura Gall-Mas,Ioannis S Pateras,Sofia D P Theodorou,Martin R J Namini,Morten B Hansen,Océane C B Martin,Chella Krishna Vadivel,Konstantinos Ntostoglou,Deborah Butter,Michael Givskov,Carsten Geisler,Arne N Akbar,Vassilis G Gorgoulis,Teresa Frisan,Niels Ødum,Thorbjørn Krejsgaard

Cancers 12: PubMed32645996

2020

Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPα Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandros Papalampros,Michail Vailas,Konstantinos Ntostoglou,Maria Lopez Chiloeches,Stratigoula Sakellariou,Niki V Chouliari,Menelaos G Samaras,Paraskevi D Veltsista,Sofia D P Theodorou,Aggelos T Margetis,Anna Bergonzini,Lysandros Karydakis,Natasha Hasemaki,Sophia Havaki,Ioannis I Moustakas,Antonios Chatzigeorgiou,Timokratis Karamitros,Eleni Patsea,Christos Kittas,Andreas C Lazaris,Evangelos Felekouras,Vassilis G Gorgoulis,Teresa Frisan,Ioannis S Pateras

Journal of experimental & clinical cancer research 37:136 PubMed29973237

2018

Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Guozhen Cui,Robert C Martin,Hang Jin,Xingkai Liu,Harshul Pandit,Hengjun Zhao,Lu Cai,Ping Zhang,Wei Li,Yan Li
View all publications
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