JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183290

TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green)

4

(1 Review)

|

(1 Publication)

TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & HRP/Green)( ab183290) is designed to be used with supplied 1x mouse, 1x rabbit and 1x goat primary antibodies on human tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Goat IgG, AP Polymer anti-Rabbit IgG and HRP Polymer anti-Mouse IgG and three distinct chromogens: DAB (Brown), Permanent Red (Red) and Emerald Chromogen (Green)
- Designed to prevent non-specific binding of endogenous biotin
- Non-Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Assay type

Polymer Multiplex

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's TripleStain IHC Kit (ab183290) is designed to be used with user-supplied one goat, one mouse and one rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissur; however it may also be used on frozen or freshly prepared monolayer cell smears.

Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183290) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183290) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the user to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.

style
left-column

What's included?

{ "values": { "60mL": { "sellingSize": "60 mL", "publicAssetCode":"ab183290-60mL", "assetComponentDetails": [ { "size":"1 x 60 mL", "name":"Triple Stain Blocker", "number":"AB183290-CMP12" }, { "size":"1 x 60 mL", "name":"Rabbit AP Polymer", "number":"AB183290-CMP11" }, { "size":"1 x 120 mL", "name":"Permanent Red Substrate", "number":"AB183290-CMP09" }, { "size":"1 x 24 mL", "name":"Permanent Red Activator (5x)", "number":"AB183290-CMP08" }, { "size":"1 x 60 mL", "name":"Mouse HRP Polymer", "number":"AB183290-CMP06" }, { "size":"1 x 60 mL", "name":"Goat HRP Polymer", "number":"AB183290-CMP05" }, { "size":"1 x 60 mL", "name":"Emerald Chromogen", "number":"AB183290-CMP04" }, { "size":"1 x 120 mL", "name":"DAB Substrate", "number":"AB183290-CMP03" }, { "size":"1 x 6 mL", "name":"DAB Chromogen (20x)", "number":"AB183290-CMP02" }, { "size":"1 x 1.2 mL", "name":"Permanent Red Chromogen (100x)", "number":"AB183290-CMP01" } ] }, "6mL": { "sellingSize": "6 mL", "publicAssetCode":"ab183290-6mL", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Triple Stain Blocker", "number":"AB183290-CMP12" }, { "size":"1 x 6 mL", "name":"Rabbit AP Polymer", "number":"AB183290-CMP11" }, { "size":"1 x 15 mL", "name":"Permanent Red Substrate", "number":"AB183290-CMP09" }, { "size":"1 x 3 mL", "name":"Permanent Red Activator (5x)", "number":"AB183290-CMP08" }, { "size":"1 x 6 mL", "name":"Non-aqueous Mounting Medium", "number":"AB183290-CMP07" }, { "size":"1 x 6 mL", "name":"Mouse HRP Polymer", "number":"AB183290-CMP06" }, { "size":"1 x 6 mL", "name":"Goat HRP Polymer", "number":"AB183290-CMP05" }, { "size":"1 x 6 mL", "name":"Emerald Chromogen", "number":"AB183290-CMP04" }, { "size":"1 x 12 mL", "name":"DAB Substrate", "number":"AB183290-CMP03" }, { "size":"1 x 1.5 mL", "name":"DAB Chromogen (20x)", "number":"AB183290-CMP02" }, { "size":"1 x 150 µL", "name":"Permanent Red Chromogen (100x)", "number":"AB183290-CMP01" } ] }, "18mL": { "sellingSize": "18 mL", "publicAssetCode":"ab183290-18mL", "assetComponentDetails": [ { "size":"1 x 36 mL", "name":"DAB Substrate", "number":"AB183290-CMP03", "productcode":"" }, { "size":"1 x 18 mL", "name":"Triple Stain Blocker", "number":"AB183290-CMP12", "productcode":"" }, { "size":"1 x 18 mL", "name":"Rabbit AP Polymer", "number":"AB183290-CMP11", "productcode":"" }, { "size":"1 x 36 mL", "name":"Permanent Red Substrate", "number":"AB183290-CMP09", "productcode":"" }, { "size":"1 x 7.2 mL", "name":"Permanent Red Activator (5x)", "number":"AB183290-CMP08", "productcode":"" }, { "size":"1 x 18 mL", "name":"Non-aqueous Mounting Medium", "number":"AB183290-CMP07", "productcode":"" }, { "size":"1 x 18 mL", "name":"Mouse HRP Polymer", "number":"AB183290-CMP06", "productcode":"" }, { "size":"1 x 18 mL", "name":"Goat HRP Polymer", "number":"AB183290-CMP05", "productcode":"" }, { "size":"1 x 18 mL", "name":"Emerald Chromogen", "number":"AB183290-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"DAB Chromogen (20x)", "number":"AB183290-CMP02", "productcode":"" }, { "size":"1 x 360 µL", "name":"Permanent Red Chromogen (100x)", "number":"AB183290-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0266532 PubMed35421154

2022

Fused toes homolog, a potential molecular regulator of human papillomavirus type 16 E6 and E7 oncoproteins in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Prabakaran D S,Pankaj Kumar Chaturvedi,Dineshkumar Krishnamoorthy,Young-Seok Seo,Mallikarjuna Thippana,Woo-Yoon Park
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Reagents, Controls & Accessories

AB2601

PAP pen

Schematic Diagram - PAP pen (AB2601)

  • 5
  • 4
Sample Prep & Detection Kits

AB183286

TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green)

Immunohistochemistry paraffin embedded sections - TripleStain IHC Kit: M&M&R on Human tissue (DAB, AP/Red & HRP/Green) (AB183286)

  • 5
  • 1
Sample Prep & Detection Kits

AB64261

Rabbit specific HRP/DAB Detection IHC Kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit specific HRP/DAB Detection IHC Kit (AB64261)

  • 5
  • 5
Sample Prep & Detection Kits

AB183297

TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)

  • 5
  • 1
Sample Prep & Detection Kits

AB64264

Mouse and Rabbit Specific HRP/DAB Detection IHC kit

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse and Rabbit Specific HRP/DAB Detection IHC kit (AB64264)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com