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AB183288

TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP)

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TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & HRP/Green)( ab183290) is designed to be used with supplied 1x mouse, 2x rabbit primary antibodies on human tissue or cell samples. Suitable with formalin-fixed, paraffin-embedded (FFPE) or frozen tissue.

- Contains a HRP Polymer anti-Rabbit IgG, AP Polymer anti-Rabbit IgG and HRP Polymer anti-Mouse IgG and three distinct chromogens: DAB (Brown), Permanent Red (Red) and Emerald Chromogen (Green)
- Designed to prevent non-specific binding of endogenous biotin
- Non-Aqueous Mounting Medium provided
- Available in different sizes to fit your experimental needs

Key facts

Assay type

Polymer Multiplex

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.

Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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