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Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples.

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Assay type

Polymer Multiplex

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

What's included?

60 mL
Components
Antibody Blocker (40x)
1 x 100 mL
Blocker A
1 x 60 mL
Blocker B
1 x 60 mL
DAB Chromogen (20x)
1 x 6 mL
DAB Substrate
1 x 120 mL
Emerald Chromogen
1 x 60 mL
Mouse HRP Polymer
1 x 60 mL
Permanent Red Activator (5x)
1 x 24 mL
Permanent Red Chromogen (100x)
1 x 1.2 mL
Permanent Red Substrate
1 x 120 mL
Rabbit AP Polymer
1 x 60 mL
Rabbit HRP Polymer
1 x 60 mL

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Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples.

Key facts

Assay type

Polymer Multiplex

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's TripleStain IHC Kit (ab183288) is designed to be used with one mouse and two rabbit primary antibodies, supplied by the user, to detect three distinct antigens on human tissue or cell samples. This kit has been tested on paraffin embedded tissue specimens, however, it can also be used for frozen tissue or cell smears.

Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183288) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit AP Polymer and Rabbit HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and Emerald (green). Abcam's TripleStain IHC Kit (ab183288) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using our unique blocking system. Simplified steps allow users to complete triple staining within 5 hours (without antigen retrieval) or 6 hours (with antigen retrieval). The well tested protocol provides the user with the ability to permanently mount slides with a coverslip.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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