JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183295

TripleStain IHC Kit: R&R&M on rodent tissue (DAB , AP/Red & HRP/Green)

Be the first to review this product! Submit a review

|

(1 Publication)

Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample.

Key facts

Assay type

Polymer Multiplex

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's TripleStain IHC Kit (ab183295) is designed to be uses with user-supplied one mouse and two rabbit primary antibodies to detect three distinct antigens on a single mouse/rat tissue or cell sample. This kit has been tested on tissue specimens that are paraffin embedded; however it may also be used on frozen or freshly prepared monolayer cell smears.

Triple staining uses traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183295) supplies polymer enzyme conjugates: Mouse HRP Polymer, Rabbit HRP Polymer and Rabbit AP Polymer with three substrates/chromogens; DAB (brown), Emerald (green), and Permanent Red (Red). Abcam's TripleStain IHC Kit (ab183295) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross detection when detecting two primary antibodies from the same host species using a unique blocking system. Simplified steps allow the user to complete triple staining within 5 hours (without antigen retrieval) or 6-7 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with coverslip.

style
left-column

What's included?

{ "values": { "18mL": { "sellingSize": "18 mL", "publicAssetCode":"ab183295-18mL", "assetComponentDetails": [ { "size":"1 x 18 mL", "name":"Rabbit HRP Polymer", "number":"AB183295-CMP15" }, { "size":"1 x 18 mL", "name":"Rabbit AP Polymer", "number":"AB183295-CMP14" }, { "size":"1 x 36 mL", "name":"Permanent Red Substrate", "number":"AB183295-CMP12" }, { "size":"1 x 7.2 mL", "name":"Permanent Red Activator (5x)", "number":"AB183295-CMP11" }, { "size":"1 x 18 mL", "name":"Non-aqueous Mounting Medium", "number":"AB183295-CMP10" }, { "size":"1 x 18 mL", "name":"Mouse Primer", "number":"AB183295-CMP09" }, { "size":"1 x 18 mL", "name":"Mouse HRP Polymer", "number":"AB183295-CMP08" }, { "size":"1 x 18 mL", "name":"Emerald Chromogen", "number":"AB183295-CMP07" }, { "size":"1 x 36 mL", "name":"DAB Substrate", "number":"AB183295-CMP06" }, { "size":"1 x 2 mL", "name":"DAB Chromogen (20x)", "number":"AB183295-CMP05" }, { "size":"1 x 18 mL", "name":"Blocker B", "number":"AB183295-CMP04" }, { "size":"1 x 18 mL", "name":"Blocker A", "number":"AB183295-CMP03" }, { "size":"1 x 360 µL", "name":"Permanent Red Chromogen (100x)", "number":"AB183295-CMP02" }, { "size":"1 x 50 mL", "name":"Antibody Blocker (40x)", "number":"AB183295-CMP01" } ] }, "6mL": { "sellingSize": "6 mL", "publicAssetCode":"ab183295-6mL", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Rabbit HRP Polymer", "number":"AB183295-CMP15" }, { "size":"1 x 6 mL", "name":"Rabbit AP Polymer", "number":"AB183295-CMP14" }, { "size":"1 x 15 mL", "name":"Permanent Red Substrate", "number":"AB183295-CMP12" }, { "size":"1 x 3 mL", "name":"Permanent Red Activator (5x)", "number":"AB183295-CMP11" }, { "size":"1 x 6 mL", "name":"Non-aqueous Mounting Medium", "number":"AB183295-CMP10" }, { "size":"1 x 6 mL", "name":"Mouse Primer", "number":"AB183295-CMP09" }, { "size":"1 x 6 mL", "name":"Mouse HRP Polymer", "number":"AB183295-CMP08" }, { "size":"1 x 6 mL", "name":"Emerald Chromogen", "number":"AB183295-CMP07" }, { "size":"1 x 12 mL", "name":"DAB Substrate", "number":"AB183295-CMP06" }, { "size":"1 x 1.5 mL", "name":"DAB Chromogen (20x)", "number":"AB183295-CMP05" }, { "size":"1 x 6 mL", "name":"Blocker B", "number":"AB183295-CMP04" }, { "size":"1 x 6 mL", "name":"Blocker A", "number":"AB183295-CMP03" }, { "size":"1 x 150 µL", "name":"Permanent Red Chromogen (100x)", "number":"AB183295-CMP02" }, { "size":"1 x 30 mL", "name":"Antibody Blocker (40x)", "number":"AB183295-CMP01" } ] }, "60mL": { "sellingSize": "60 mL", "publicAssetCode":"ab183295-60mL", "assetComponentDetails": [ { "size":"1 x 60 mL", "name":"Rabbit HRP Polymer", "number":"AB183295-CMP15", "productcode":"" }, { "size":"1 x 60 mL", "name":"Rabbit AP Polymer", "number":"AB183295-CMP14", "productcode":"" }, { "size":"1 x 120 mL", "name":"Permanent Red Substrate", "number":"AB183295-CMP12", "productcode":"" }, { "size":"1 x 24 mL", "name":"Permanent Red Activator (5x)", "number":"AB183295-CMP11", "productcode":"" }, { "size":"1 x 60 mL", "name":"Mouse Primer", "number":"AB183295-CMP09", "productcode":"" }, { "size":"1 x 60 mL", "name":"Mouse HRP Polymer", "number":"AB183295-CMP08", "productcode":"" }, { "size":"1 x 60 mL", "name":"Emerald Chromogen", "number":"AB183295-CMP07", "productcode":"" }, { "size":"1 x 120 mL", "name":"DAB Substrate", "number":"AB183295-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"DAB Chromogen (20x)", "number":"AB183295-CMP05", "productcode":"" }, { "size":"1 x 60 mL", "name":"Blocker B", "number":"AB183295-CMP04", "productcode":"" }, { "size":"1 x 60 mL", "name":"Blocker A", "number":"AB183295-CMP03", "productcode":"" }, { "size":"1 x 1.2 mL", "name":"Permanent Red Chromogen (100x)", "number":"AB183295-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"Antibody Blocker (40x)", "number":"AB183295-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical neuroscience 14:103-116 PubMed37346877

2023

Datumetine Preferentially Upregulates N-methyl-D-aspartate Receptor Signalling Pathways in Different Brain Regions of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Azeez Olakunle Ishola,Adedeji Enitan Adetunji,Isaac Chukwunwike Abanum,Adesola Akorede Adeyemi,Caleb Kenechukwu Faleye,Jane Babale Martins,Nnenna Chimdalu Ogbe,Toluwalase Cherish Ogundipe,Karen Ezichi Okewulonu,Umo Emmanuel Okon,Deborah Irenoise Ovbude,Richard Yomi Akele,Naeemat Tolulope Omotade,Moyosore Salihu Ajao
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Sample Prep & Detection Kits

AB183297

TripleStain IHC Kit: M&R&Rt on rodent tissue (DAB, HRP/Green & AP/Red)

  • 5
  • 1
Sample Prep & Detection Kits

AB183292

TripleStain IHC kit: M&M&R on rodent tissue (DAB, HRP/Green & AP/Red)

  • 0
  • 0
Primary Antibodies

AB7753

Anti-pan Cytokeratin antibody [C-11]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-pan Cytokeratin antibody [C-11] (AB7753)

  • 4
  • 23
Sample Prep & Detection Kits

AB183288

TripleStain IHC Kit: R&R&M on human tissue (DAB, AP/Red & Green/HRP)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com