Universal HIER antigen retrieval reagent (10X)

• IHC

Unknown

Immunohistochemistry - Universal HIER antigen retrieval reagent (10X) (AB208572)

Human tonsil stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Universal HIER antigen retrieval reagent (10X) (AB208572)

Tissue Microarrays stained for Anti-PD-L1 antibody [28-8] using ab205921 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent). The sections were incubated with ab205921 at +4°C overnight. For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is recommended.

• IHC

Unknown

Immunohistochemistry - Universal HIER antigen retrieval reagent (10X) (AB208572)

Human melanoma stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).

• IHC

Unknown

Immunohistochemistry - Universal HIER antigen retrieval reagent (10X) (AB208572)

Human tonsil stained with PD-L1 RabMAb antibody (clone 28-8, ab205921) using Universal HIER antigen retrieval reagent (ab208572).

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Universal HIER antigen retrieval reagent (10X) (AB208572)

Immunostaining for TNC within the blood vessel. Human lung samples were formalin-fixed and paraffin-embedded (FFPE). Deparaffinized and rehydrated 5-micron sections were subjected to antigen heat retrieval using citrate buffer (ab208572), for 30 min at 100 °C, cooled to room temperature for 20 min, treated with 3% hydrogen peroxide for 5 min, blocked for 1 h , and probed with primary antibody (TNC, 1 : 500, ab108930) overnight in 10% blocking solution. Scale bar represents 100 μm

Image from Herrera, JA et al., Clin Proteomics. 17:24. doi: 10.1186/s12014-020-09287-6. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Universal HIER antigen retrieval reagent (10X) (AB208572)

Immunohistochemistry of various tumor markers on brain sections. Slides were immersed in 1X universal antigen retrieval reagent (ab208572) and incubated for 20 min at 95 °C. Slides were stained to detect the expression of PRDX1 (ab41906), γ-H2A.X ( Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (ab 15580), 53BP1 (ab21083) and cleaved caspase 3 (ab13847) using an HRP/DAB detection method by using a mouse and rabbit specific HRP/DAB detection IHC kit (ab80436), following manufacturers recommendations with minor modifications.

Image from Sajesh et al., 11(9):481. doi: 10.3390/pharmaceutics11090481. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/