Abcam's Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers.
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Abcam's Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers.
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Abcam's Universal HIER antigen retrieval reagent (10X) is a heat-induced epitope retrieval (HIER) solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers, including citrate buffer, EDTA or high pH Tris buffers. Antibody titers are doubled or tripled when compared to citrate buffer, pH 6.0. Universal HIER antigen retrieval reagent (10X) incorporates a color-coded high temperature pH indicator solution, allowing the end-user to check that the solution is at the correct dilution and pH by visual inspection. This product is specially formulated for superior pH stability at high temperatures in order to help prevent the possibility of losing pH-sensitive antigens. Universal HIER antigen retrieval reagent (10X) is non-toxic, non-flammable, odorless and sodium azide- and thimerosal-free.
ab208572 is the recommended HIER antigen retrieval reagent for PD-L1 RabMAb (clone 28-8, Anti-PD-L1 antibody [28-8] ab205921).
The Universal HIER antigen retrieval reagent is a buffered solution that is intended for laboratory use in heat induced antigen retrieval of formalin-fixed paraffin-embedded (FFPE) tissues for immunohistochemistry (IHC) procedures. The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
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Human tonsil stained with PD-L1 RabMAb antibody (clone 28-8, Anti-PD-L1 antibody [28-8] ab205921) using Universal HIER antigen retrieval reagent (ab208572).
Immunohistochemistry of various tumor markers on brain sections. Slides were immersed in 1X universal antigen retrieval reagent (ab208572) and incubated for 20 min at 95 °C. Slides were stained to detect the expression of PRDX1 (Anti-Peroxiredoxin 1/PAG antibody ab41906), γ-H2A.X ( Anti-γ-H2A.X phosphorylated S139 antibody; Anti-gamma H2A.X (phospho S139) antibody ab2893), Ki 67 (ab 15580), 53BP1 (Anti-53BP1 antibody ab21083) and cleaved caspase 3 (Anti-Caspase-3 antibody ab13847) using an HRP/DAB detection method by using a mouse and rabbit specific HRP/DAB detection IHC kit (ab80436), following manufacturers recommendations with minor modifications.
Human tonsil stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).
Immunostaining for TNC within the blood vessel. Human lung samples were formalin-fixed and paraffin-embedded (FFPE). Deparaffinized and rehydrated 5-micron sections were subjected to antigen heat retrieval using citrate buffer (ab208572), for 30 min at 100 °C, cooled to room temperature for 20 min, treated with 3% hydrogen peroxide for 5 min, blocked for 1 h , and probed with primary antibody (TNC, 1:500, Anti-Tenascin C antibody [EPR4219] ab108930) overnight in 10% blocking solution. Scale bar represents 100 μm
Human melanoma stained with rabbit monoclonal [28-8] to PD-L1 using Universal HIER antigen retrieval reagent (ab208572).
Tissue Microarrays stained for Anti-PD-L1 antibody [28-8] using Anti-PD-L1 antibody [28-8] ab205921 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent). The sections were incubated with Anti-PD-L1 antibody [28-8] ab205921 at +4°C overnight. For IHC detection kit, Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) is recommended.
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