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Complex I Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Enzyme Activity in Mouse, Rat, Cow, Human in Cell culture extracts, Tissue, Cell culture supernatant samples.

Sample types

Cell culture extracts, Tissue, Cell culture supernatant

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Reactivity data

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

48 Test
Components
10X Blocking Buffer
1 x 400 µL
96-well microplate
2 x 1 Unit
Dipsticks
1 x 48 Unit
Extraction Buffer (ab260490)
1 x 15 mL
NADH (lyophilized)
1 x 1 Vial
NBT (Lyophilized) - 10mg
1 x 1 Vial
Reaction Buffer
1 x 15 mL
Wash buffer
1 x 2 mL

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Complex I Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Enzyme Activity in Mouse, Rat, Cow, Human in Cell culture extracts, Tissue, Cell culture supernatant samples.

Key facts

Sample types

Cell culture extracts, Tissue, Cell culture supernatant

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Contains 48 dipsticks and necessary components to quantify the activity of the Complex I enzyme complex in human, bovine, rat and mouse samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
In this assay the specificity of anti-Complex I monoclonal antibodies (mAbs) is combined with the well-characterized Complex I in-gel activity assay that is not rotenone sensitive. First, Complex I is immunocaptured (i.e. immuno-precipitated in active form) on the dipstick. Second, the dipstick is immersed in Complex I activity buffer solution containing NADH as a substrate and nitrotetrazolium blue (NBT) as the electron acceptor. Immunocaptured Complex I oxidizes NADH and the resulting H+ reduces NBT to form a blue-purple precipitate at the Complex I antibody line on the dipstick. The signal intensity of this precipitate corresponds to the level of Complex I enzyme activity in the sample. Combined with dipstick assay kit for measuring Complex I quantity (ab109722/MS131 for human sample; ab109875/MS133 for rodent samples), it is possible to determine the relative specific activity of immunocaptured Complex I. The signal intensity is best measured by a dipstick reader or may be analyzed by a standard imaging system.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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