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AB109722

Complex I Human Protein Quantity Dipstick Assay Kit

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(9 Publications)

Complex I Human Protein Quantity Dipstick Assay Kit immunoassay kit for the detection of Complex I Human Protein Quantity Dipstick Assay Kit in Cow, Human in Cell/Tissue Extracts samples.

View Alternative Names

CIA30, CGI-65, NDUFAF1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1

3 Images
ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)
  • ELISA

Supplier Data

ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)

An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.

ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)
  • ELISA

Supplier Data

ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)

An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.

Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)
  • sELISA

Supplier Data

Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (AB109722)

Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Key facts

Sample types

Cell culture extracts, Tissue

Reacts with

Cow, Human

Assay type

Sandwich (quantitative)

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab109722 contains 48 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with ab109720 to determine the relative specific activity of Complex I.

All components are stable in their provided containers at room temperature out of direct sunlight.

After diluting the 10X Blocking Buffer to 2X, store at 4°C. For long-term storage, all buffers can be stored at 4°C.

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What's included?

{ "values": { "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab109722-48Test", "assetComponentDetails": [ { "size":"1 x 30 Test", "name":"Gold-conjugated antibody (dried in microplate wells)", "number":"AB109722-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Wash buffer", "number":"AB109722-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB109722-CMP03", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Buffer B (10X Blocking solution)", "number":"AB109722-CMP05", "productcode":"" }, { "size":"1 x 30 Unit", "name":"Dipsticks", "number":"AB109722-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex I also known as NADH dehydrogenase or NADH:ubiquinone oxidoreductase is a large enzyme complex with a molecular mass of approximately 1000 kDa. It is expressed in the inner mitochondrial membrane of eukaryotic cells. As the first enzyme in the mitochondrial respiratory chain Complex I plays a critical mechanical role in cellular respiration. It transfers electrons from NADH to ubiquinone coupled with the translocation of protons across the inner mitochondrial membrane contributing to the generation of a proton gradient used to produce ATP.
Biological function summary

Complex I acts as an integral component of the mitochondrial respiratory chain which is a series of protein complexes involved in cellular energy production. As part of this complex system Complex I is essential for effective oxidative phosphorylation. Its activity is assessed using protein activity assays including immunocapture or complex activity assays and microplate assays. Complex I activity influences the overall efficiency of ATP production affecting energy-dependent cellular processes.

Pathways

Complex I functions within the electron transport chain one of the major pathways in cellular respiration. This pathway is vital for ATP synthesis providing the energy currency required by cells. Complex I works closely with other electron transport chain complexes such as Complex II (succinate dehydrogenase complex) and Complex III (cytochrome c reductase) to drive oxidation-reduction reactions and maintain cellular metabolism.

Defects in Complex I are linked with mitochondrial diseases and neurodegenerative disorders such as Leigh syndrome and Parkinson's disease. Mutations in Complex I subunits disrupt normal electron transport and ATP production leading to increased oxidative stress and neuronal cell damage. Other mitochondrial proteins such as cytochrome c oxidase are also implicated in these conditions emphasizing the interconnected nature of mitochondrial dysfunction in disease progression.
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Product protocols

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Target data

As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
See full target information NDUFAF1

Additional targets

NDUFAF2,AIFM1,NDUFS4,ECSIT,NDUFAF4,NDUFAF2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience 594:1-10 PubMed41397672

2025

Monoacylglycerol lipase inhibition improves motor function and reduces oxidative stress in hemiparkinsonian mice.

Applications

Unspecified application

Species

Unspecified reactive species

Delia Maciel Mendoza-Camacho,Juan Manuel Viveros-Paredes,Mario Eduardo Flores-Soto,Alma Hortensia Martínez-Preciado,Aldo Rafael Tejeda-Martínez

Research in veterinary science 95:870-8 PubMed24099743

2013

Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.

Applications

FuncS

Species

Horse

Justine D Ceusters,Ange A Mouithys-Mickalad,Thierry J Franck,Ginette P Deby-Dupont,Sandrine Derochette,Didier A Serteyn

Mechanisms of ageing and development 134:460-6 PubMed24012633

2013

Helicobacter pylori infection affects mitochondrial function and DNA repair, thus, mediating genetic instability in gastric cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Manuel Dantas Machado,Claus Desler,Sisse Bøggild,Jesper A B Strickertsson,Lennart Friis-Hansen,Ceu Figueiredo,Raquel Seruca,Lene Juel Rasmussen

The Journal of clinical investigation 120:1749-61 PubMed20364084

2010

Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Ye,Suh Young Jeong,Manik C Ghosh,Gennadiy Kovtunovych,Laura Silvestri,Danilo Ortillo,Naoya Uchida,John Tisdale,Clara Camaschella,Tracey A Rouault

Journal of proteome research 9:2390-401 PubMed20334438

2010

Differential effects of wild-type and A53T mutant isoform of alpha-synuclein on the mitochondrial proteome of differentiated SH-SY5Y cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyla Pennington,Jianhe Peng,Chao-Chun Hung,Rosamonde E Banks,Philip A Robinson

Toxicology and applied pharmacology 238:150-9 PubMed19442681

2009

Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Rohini Kashimshetty,Varsha G Desai,Vijay M Kale,Taewon Lee,Carrie L Moland,William S Branham,Lee S New,Eric C Y Chan,Husam Younis,Urs A Boelsterli

Journal of immunological methods 343:1-12 PubMed19152798

2009

Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sashi Nadanaciva,John H Willis,Melissa L Barker,Dima Gharaibeh,Roderick A Capaldi,Michael F Marusich,Yvonne Will

The Journal of biological chemistry 284:2307-19 PubMed19036724

2008

Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Kristel Vercauteren,Natalie Gleyzer,Richard C Scarpulla

AIDS research and human retroviruses 24:1255-62 PubMed18844460

2008

Mitochondrial oxidative phosphorylation protein levels in peripheral blood mononuclear cells correlate with levels in subcutaneous adipose tissue within samples differing by HIV and lipoatrophy status.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia M Shikuma,Mariana Gerschenson,Dominic Chow,Daniel E Libutti,John H Willis,James Murray,Roderick A Capaldi,Michael Marusich
View all publications
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