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Complex I Human Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Human Protein Quantity in Cow, Human in Cell culture extracts, Tissue samples.

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Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

What's included?

48 Test
Components
Buffer B (10X Blocking solution)
1 x 0.4 mL
Dipsticks
1 x 30 Unit
Extraction Buffer (ab260490)
1 x 15 mL
Gold-conjugated antibody (dried in microplate wells)
1 x 30 Test
Wash buffer
1 x 2 mL

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Complex I Human Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Human Protein Quantity in Cow, Human in Cell culture extracts, Tissue samples.

Key facts

Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Cow, Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab109722 contains 48 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.

Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with ab109720 to determine the relative specific activity of Complex I.

All components are stable in their provided containers at room temperature out of direct sunlight.

After diluting the 10X Blocking Buffer to 2X, store at 4°C. For long-term storage, all buffers can be stored at 4°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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