Complex I Human Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Human Protein Quantity in Cow, Human in Cell culture extracts, Tissue samples.
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- Research in veterinary science 95:870-82013Effect of different kinds of anoxia/reoxygenation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts.
- Mechanisms of ageing and development 134:460-62013Helicobacter pylori infection affects mitochondrial function and DNA repair, thus, mediating genetic instability in gastric cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Manuel Dantas Machado et. al.PubMed 24012633
- The Journal of clinical investigation 120:1749-612010Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Ye et. al.PubMed 20364084
- Journal of proteome research 9:2390-4012010Differential effects of wild-type and A53T mutant isoform of alpha-synuclein on the mitochondrial proteome of differentiated SH-SY5Y cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyla Pennington,Jianhe Peng,Chao-Chun Hung,Rosamonde E Banks,Philip A RobinsonPubMed 20334438
- Toxicology and applied pharmacology 238:150-92009Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRohini Kashimshetty et. al.PubMed 19442681
- Journal of immunological methods 343:1-122009Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSashi Nadanaciva et. al.PubMed 19152798
- The Journal of biological chemistry 284:2307-192008Short hairpin RNA-mediated silencing of PRC (PGC-1-related coactivator) results in a severe respiratory chain deficiency associated with the proliferation of aberrant mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristel Vercauteren,Natalie Gleyzer,Richard C ScarpullaPubMed 19036724
- AIDS research and human retroviruses 24:1255-622008Mitochondrial oxidative phosphorylation protein levels in peripheral blood mononuclear cells correlate with levels in subcutaneous adipose tissue within samples differing by HIV and lipoatrophy status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia M Shikuma,Mariana Gerschenson,Dominic Chow,Daniel E Libutti,John H Willis,James Murray,Roderick A Capaldi,Michael MarusichPubMed 18844460
Key facts
- Sample types
- Cell culture extracts, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow, Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Additional Targets
NDUFAF2, AIFM1, NDUFS4, ECSIT, NDUFAF4, NDUFAF2
Alternative names
CIA30, CGI-65, NDUFAF1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1
What's included?
Recommended products
Complex I Human Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Complex I Human Protein Quantity in Cow, Human in Cell culture extracts, Tissue samples.
Key facts
- Sample types
- Cell culture extracts, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Cow, Human
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ab109722 contains 48 dipsticks and necessary components to quantify the levels of the fully assembled Complex I enzyme complex in human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the Complex I enzyme complex. One antibody is immobilized on the nitrocellulose membrane in a thin line perpendicular to the length of the dipstick, while the other is gold-conjugated and combined with the sample mix. The sample contents containing the gold-conjugated mAb wick past the mAb immobilized on the dipstick. When assembled Complex I is present in the sample, a red line appears at the site of the anti-Complex I antibody line. The signal intensity is directly related to the amount of Complex I in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system. To identify defects in Complex I that do not affect enzyme assembly combine this assay with Complex I Enzyme Activity Dipstick Assay Kit ab109720 to determine the relative specific activity of Complex I.
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C. For long-term storage, all buffers can be stored at 4°C.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complex I also known as NADH dehydrogenase or NADH:ubiquinone oxidoreductase is a large enzyme complex with a molecular mass of approximately 1000 kDa. It is expressed in the inner mitochondrial membrane of eukaryotic cells. As the first enzyme in the mitochondrial respiratory chain Complex I plays a critical mechanical role in cellular respiration. It transfers electrons from NADH to ubiquinone coupled with the translocation of protons across the inner mitochondrial membrane contributing to the generation of a proton gradient used to produce ATP.
- Biological function summary
Complex I acts as an integral component of the mitochondrial respiratory chain which is a series of protein complexes involved in cellular energy production. As part of this complex system Complex I is essential for effective oxidative phosphorylation. Its activity is assessed using protein activity assays including immunocapture or complex activity assays and microplate assays. Complex I activity influences the overall efficiency of ATP production affecting energy-dependent cellular processes.
- Pathways
Complex I functions within the electron transport chain one of the major pathways in cellular respiration. This pathway is vital for ATP synthesis providing the energy currency required by cells. Complex I works closely with other electron transport chain complexes such as Complex II (succinate dehydrogenase complex) and Complex III (cytochrome c reductase) to drive oxidation-reduction reactions and maintain cellular metabolism.
- Associated diseases and disorders
Defects in Complex I are linked with mitochondrial diseases and neurodegenerative disorders such as Leigh syndrome and Parkinson's disease. Mutations in Complex I subunits disrupt normal electron transport and ATP production leading to increased oxidative stress and neuronal cell damage. Other mitochondrial proteins such as cytochrome c oxidase are also implicated in these conditions emphasizing the interconnected nature of mitochondrial dysfunction in disease progression.
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3 product images
ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
Figure 2. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
Figure 1. An example using ab109722 to quantify Complex I levels using various concentration of human fibroblast extract.
Sandwich ELISA - Complex I Human Protein Quantity Dipstick Assay Kit (ab109722)
Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.
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