Frataxin Protein Quantity Dipstick Assay Kit (ab109881)

Frataxin Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Frataxin Protein Quantity in Human in Whole Blood samples.

This product has no reviews yet! Submit a review

Alternative names=Friedreich ataxia protein, Fxn, FXN, FRDA, X25

Sample types: Whole Blood
Assay type: Sandwich (quantitative)
Reactive species: Human

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

See full target information FXN

Function

Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.

What's included?

30 Test

Components

Buffer B (10X Blocking solution)

1 x 0.4 mL

Dipsticks

1 x 30 Unit

Extraction Buffer (ab260490)

1 x 15 mL

Gold-conjugated antibody (dried in microplate wells)

1 x 30 Test

Wash buffer

1 x 2 mL

Recommended products

Frataxin Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Frataxin Protein Quantity in Human in Whole Blood samples.

Alternative names=Friedreich ataxia protein, Fxn, FXN, FRDA, X25

Key facts

Sample types: Whole Blood
Assay type: Sandwich (quantitative)
Reactive species: Human
Assay Platform: Reagents

Storage

Shipped at conditions: Blue Ice
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

ab109881 is used to rapidly quantify frataxin protein levels from human sample materials. Purification of mitochondria is not necessary for the performance of this assay. Based on the immunologic sandwich assay, the kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the mature form of frataxin. One antibody is immobilized on the nitrocellulose membrane of the dipstick in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated which gives a visual signal. When frataxin is present in the sample, a red-colored line appears on the dipstick at the site of the anti-frataxin mAb immobilized on the membrane. The signal intensity is directly related to the level of frataxin in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.

All components are stable in their provided containers at room temperature out of direct sunlight.

After diluting the 10X Blocking Buffer to 2X, store at 4°C.

For long-term storage, all buffers can be stored at 4°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
Visit troubleshooting

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Products

Resources

Support