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Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Enzyme Activity in Mouse, Rat, Cow, Human, Pig in Cell culture extracts, Tissue samples.

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Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat, Cow, Human, Pig

Reactivity data

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

30 Test
Components
20X Reagent Mix
1 x 600 µL
96-well microplate
2 x 1 Unit
Blocking solution
1 x 2 mL
Detergent
1 x 1 mL
Diaphorase
1 x 400 µL
Dipsticks
1 x 30 Test
NBT
1 x 40 µL
Sample buffer
1 x 30 mL

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Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Enzyme Activity in Mouse, Rat, Cow, Human, Pig in Cell culture extracts, Tissue samples.

Key facts

Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat, Cow, Human, Pig

Assay Platform

Reagents

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

ab109882 is used to quantify the activity of the PDH enzyme complex from human, bovine, mouse, rat and pig samples. This is accomplished by immunocapturing PDH with an anti-PDH antibody immobilized on a defined section of a dipstick. The enzyme complex is immunocaptured in its native form and activity is measured by the reaction scheme. PDH activity is visualized by coupling PDH-dependent production of NADH to the reduction of NBT in the presence of excess diaphorase, forming an insoluble intensely colored precipitate at the capture line. The signal intensity is measured by a dipstick reader or analyzed by other imaging systems such as a flatbed scanner.

Purified mitochondria can be used in this assay. However, homogenized tissue and whole cells can also be used without the need for mitochondrial isolation.

Pyruvate dehydrogenase activity is regulated by PDH kinase and PDH phosphatase. Cells grown in glucose media derive most of their energy from glycolysis; therefore, most of their PDH complex may be in an inactive phosphorylated form at the time of isolation. Growing cells in alternate carbon sources such as glutamine/galactose may up-regulate PDH activity. This kit does not include PDH kinases, PDH phosphatases or their respective inhibitors. These may be incorporated into the assay at the user's discretion.

Store dipsticks at room temperature out of direct sunlight in their provided desiccant container. Avoid high humidity conditions.

Store 20X Reagent Mix, Detergent, and Diaphorase at -80°C, but these may be aliquoted upon receipt to prevent freeze/thaw cycles.

Store NBT in the dark at -80°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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