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Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Protein Quantity in Mouse, Rat, Cow, Human in Cell culture extracts, Tissue samples.

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Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat, Cow, Human

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

30 Test
Components
Buffer B (10X Blocking solution)
1 x 0.4 mL
Dipsticks
1 x 30 Unit
Extraction Buffer (ab260490)
1 x 15 mL
Gold-conjugated antibody (dried in microplate wells)
1 x 30 Test
Wash buffer
1 x 2 mL

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Pyruvate dehydrogenase (PDH) Protein Quantity Dipstick Assay Kit is an immunoassay kit for the detection of Pyruvate dehydrogenase (PDH) Protein Quantity in Mouse, Rat, Cow, Human in Cell culture extracts, Tissue samples.

Key facts

Sample types

Cell culture extracts, Tissue

Assay type

Sandwich (quantitative)

Reactive species

Mouse, Rat, Cow, Human

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

ab109883 (MSP31) is used to quantify the activity of the PDH enzyme complex (pyruvate dehydrogenase) from human, bovine, mouse, and rat samples.

PDH is present in all tissues; it plays a central role in metabolism as it is a key regulatory enzyme that functions at the junction between glycolysis and the tricarboxylic acid cycle. The PDH complex is composed of multiple copies of three catalytic component enzymes; pyruvate dehydrogenase or E1 (EC 1.2.4.1), dihydrolipoamide transacetylase or E2 (EC 2.3.1.12) and dihydrolipoamide dehydrogenase or E3 (EC 1.8.1.4). Inherent to its regulatory function, a number of other proteins regulate PDH activity. One of these, dihydrolipoamide dehydrogenase-binding protein (E3Bp) is necessary for the interaction of the E2 and E3 components.

This dipstick assay kit utilizes two monoclonal antibodies. One recognizes the E2 enzyme (monoclonal antibody bound to the dipstick) and the other antibody (gold-conjugated) recognizes both the E2 and E3Bp components of the PDH complex. The signal intensity is measured by a dipstick reader or analyzed by other imaging systems such as a flatbed scanner. The kit is compatible with a variety of sample types (tissue or cell culture) from a number of different species (human, bovine, mouse, and rat).

All components are stable in their provided containers at room temperature out of direct sunlight.

After diluting the 10X Blocking Buffer to 2X, store at 4°C.

For long-term storage, all buffers can be stored at 4°C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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