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AB270537

Universal Lateral Flow Assay Kit

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(2 Publications)

Universal Lateral Flow Assay Kit (ab270537) is designed for the rapid development of lateral flow immunoassay(LFA)

- Contains LFA strips, conjugation tools, buffers and controls
- Can be used with any antibody pair
- only 30 sec hands-on time
- qualitative or quantitative results
2 Images
Sandwich ELISA - Universal Lateral Flow Assay Kit (AB270537)
  • sELISA

Supplier Data

Sandwich ELISA - Universal Lateral Flow Assay Kit (AB270537)

Example of sandwich LFA assay for the detection of CRP in serum using the Universal Lateral Flow Assay kit : Ulfa-Tag and 40nm GOLD are used to conjugate the capture and detection antibody respectively. Results were analyzed using a Qiagen ESEQuant reader.

Schematic Diagram - Universal Lateral Flow Assay Kit (AB270537)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Universal Lateral Flow Assay Kit (AB270537)

How this kit works.

The capture antibody is conjugated to Lightning-Link® Ulfa-Tag, while the detection antibody is conjugated to 40 nm Gold, both of which require only 30 seconds to set up. The capture and detection antibody are diluted and incubated with the analyte and 40 nm Gold-Biotin and then run on Universal LFA strips. Universal LFA strips consist of a nitrocellulose membrane containing a 'Test line' (T-line) of immobilized anti-Ulfa-Tag antibody, that binds the Ulfa-Tag conjugated capture antibody which further binds the analyte in complex with the Gold-detection antibody. A red T-line appears when the analyte is present, and the line intensity varies depending on the analyte concentration. Universal LFA strips also contain a 'Control-line' (C-line) that exploit the extraordinarily high affinity of streptavidin for biotin, which shows that the test is valid, and an absorbent pad to promote and control the flow of sample through the membrane.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "LFA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Develop your own lateral flow immunoassay with Universal Lateral Flow Assay Kit (ab270537). Universal Lateral Flow Assay Kit (ab270537) is designed to enable simple and rapid development of proof-of-concept sandwich lateral flow immunoassays.

The kit includes everything needed for lateral flow assay development with any pair of capture and detection antibodies. It combines universal LFA strips, Lightning-Link® and Gold conjugation technologies, buffers and controls; the kit is designed so that there is no need to either:

strip the capture antibody onto LFA strips using expensive equipment and complex procedures,

or to conjugate the detection antibody to gold with passive absorption.

The signal intensities can be qualitatively analyzed using the supplied scoring card, or for a quantitative detection, an LFA reader can be used.

This kit contains Gold conjugation kit (ab154873)

Lateral Flow assay development protocol summary

- Conjugate the capture antibody to Lightning-Link® Ulfa-Tag

- Conjugate the detector antibody to 40nm Gold

- Dilute capture and detection antibodies

- Incubate with sample

- Run on universal LFA strips

- Read using scoring card or LFA reader

How other researchers are using Universal Lateral Flow Assay Kit (ab270537)

ab270537 has been used to develop a rapid salivary LFA to detect SARS-Cov-2.

Reference: Lorenzo Azzi et al. 2020

Related and recommended products

The Universal Lateral Flow Assay Kit can be used with our carrier-free, conjugation-ready antibody pairs: browse our capture and detector antibodies

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS neglected tropical diseases 19:e0013082 PubMed40523044

2025

Mass spectrometry-based discovery and diagnostic validation of T. cruzi antigens in the urine of congenitally infected Chagas Disease patients.

Applications

Unspecified application

Species

Unspecified reactive species

Kathryn Cassels,Raghad Almofeez,Jessica Roman,Hannah Steinberg,Ahana Byne,Amanda Haymond,Freddy Tinajeros,María Del Carmen Menduiña,Edith Málaga Machaca,Manuela Verástegui,José Luis Ramírez,Lance Liotta,Robert H Gilman,Alessandra Luchini

The Journal of infection 81:e75-e78 PubMed32579988

2020

Rapid Salivary Test suitable for a mass screening program to detect SARS-CoV-2: A diagnostic accuracy study.

Applications

Unspecified application

Species

Unspecified reactive species

Lorenzo Azzi,Andreina Baj,Tiziana Alberio,Marta Lualdi,Giovanni Veronesi,Giulio Carcano,Walter Ageno,Cinzia Gambarini,Lorenzo Maffioli,Salomone Di Saverio,Daniela Dalla Gasperina,Angelo Paolo Genoni,Elias Premi,Simone Donati,Claudio Azzolini,Anna Maria Grandi,Francesco Dentali,Flavio Tangianu,Fausto Sessa,Vittorio Maurino,Lucia Tettamanti,Claudia Siracusa,Andrea Vigezzi,Elisa Monti,Valentina Iori,Domenico Iovino,Giuseppe Ietto,Paolo Antonio Grossi,Angelo Tagliabue,Mauro Fasano
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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