AB270371

ACTH Antibody Pair - BSA and Azide free

RabMAb
Recombinant
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ACTH Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of ACTH.

View Alternative Names

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH

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Sandwich ELISA - ACTH Antibody Pair - BSA and Azide free (AB270371)

sELISA

Supplier Data

Sandwich ELISA - ACTH Antibody Pair - BSA and Azide free (AB270371)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab267814), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Key facts

Reacts with

Human, Mouse, Rat

Assay type

ELISA set

Range

2.5 - 160 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

The Antibody Pair can be used to quantify ACTH. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab267814), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery^® (MSD^®)
Bead-based assays
AlphaLISA^®/AlphaScreen^®
DELFIA^® immunoassays
Simoa^® and Single Molecule Counting (SMC™) immunoassays
Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adrenocorticotropic hormone (ACTH) also known as corticotropin is a peptide hormone secreted by the pituitary gland. It is a 39-amino acid polypeptide with a molecular mass of approximately 4540 Da. ACTH is primarily expressed in the anterior pituitary gland and smaller amounts can be found in the central nervous system. Its production and secretion are tightly regulated by corticotropin-releasing hormone (CRH) through a feedback mechanism involving the hypothalamic-pituitary-adrenal (HPA) axis.

Biological function summary

Adrenocorticotropic hormone stimulates the adrenal cortex to produce and release glucocorticoids especially cortisol. ACTH is not part of a larger complex but acts as a singular hormone in its pathway. The hormone also influences the expression of certain enzymes important for steroid biosynthesis. In addition to its role in the adrenal cortex ACTH can mildly interact with melanocortin receptors on adipose tissues contributing to lipolysis and inflammatory processes.

Pathways

Adrenocorticotropic hormone functions in the HPA axis one of the body's key stress response systems. The hormone also involves in the regulation of the corticosteroid biosynthesis pathway. The primary protein it interacts with in these processes is cortisol which in turn regulates many physiological processes including immune response and metabolism. ACTH and cortisol form a feedback loop where elevated cortisol levels inhibit the release of both CRH and ACTH.

Increased or decreased levels of adrenocorticotropic hormone can result in disorders such as Cushing's disease and Addison's disease. Cushing's disease is characterized by overproduction of ACTH often due to pituitary adenomas leading to excess cortisol production. Conversely in Addison’s disease inadequate ACTH or adrenal cortex dysfunction leads to insufficient cortisol production. Both conditions highlight the disrupted feedback relationship between ACTH and cortisol resulting in significant clinical symptoms that require careful management.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information ACTH

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

ACTH Antibody Pair - BSA and Azide free

Sandwich ELISA - ACTH Antibody Pair - BSA and Azide free (AB270371)

Key facts

Reacts with

Assay type

Range

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Assay Kits

Assay Kits

Product promise