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Human ABP1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ABP1.

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Sandwich ELISA - Human ABP1 Antibody Pair - BSA and Azide free (AB309257), expandable thumbnail

Key facts

Sample types

Cell Lysate, Cell culture media, Plasma, Serum, Tissue Lysate

Assay type

ELISA set

Reactive species

Human

Range

0.08 - 10 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed:12072962, PubMed:19764817, PubMed:239684, PubMed:8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed:12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed:12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed:12072962, PubMed:239684).

Alternative names

Recommended products

Human ABP1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ABP1.

Key facts

Sample types

Cell Lysate, Cell culture media, Plasma, Serum, Tissue Lysate

Assay type

ELISA set

Reactive species

Human

Range

0.08 - 10 ng/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human ABP1 Antibody Pair - BSA and Azide free (ab309257) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human ABP1 in sandwich ELISAs and many other pair-based applications.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:


  • Sandwich ELISA

  • FRET/TR-FRET/HTRF

  • Meso Scale Discovery® (MSD®)

  • Bead-based assays

  • AlphaLISA®/AlphaScreen®

  • DELFIA® immunoassays

  • Simoa® and Single Molecule Counting (SMC™) immunoassays

  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:


  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ABP1 also known as Auxin Binding Protein 1 is a protein with a molecular mass of approximately 22 kDa. This protein primarily functions as an auxin receptor in plants mediating various cellular responses to the plant hormone auxin. ABP1 locates mainly in the endoplasmic reticulum and is present across various plant tissues. The protein has a significant affinity for auxin and triggers specific intracellular signaling cascades essential for growth and development processes.

Biological function summary

ABP1 plays an important role in controlling cell elongation and division and it works in tandem with other auxin transporters. The protein functions within a broader receptor complex interacting with other proteins to modify plant cell physiology. Its action facilitates cell wall modification which directly influences the plant's response to environmental and endogenous signals. Through these interactions ABP1 significantly contributes to regulating the plant's phototropic and gravitropic responses.

Pathways

The operation of ABP1 integrates into the auxin signaling pathway and is associated with the regulation of gene expression. It links to TIR1 an important F-box protein in the SCF complex involved in the auxin signal transduction pathway. Together ABP1 and TIR1 coordinate to modulate the stability of transcription factors affecting downstream gene expression necessary for plant growth and development.

Associated diseases and disorders

ABP1 itself mostly pertains to plant biology but its function offers insights into plant growth abnormalities. Disruptions in ABP1 function may result in disorders like auxin-insensitive phenotypes leading to defective root and shoot development. Additionally ABP1's interaction with PIN proteins responsible for auxin efflux creates potential for research into correcting growth defects in crops making it a critical focus in agricultural science.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human ABP1 Antibody Pair - BSA and Azide free (ab309257), expandable thumbnail

    Sandwich ELISA - Human ABP1 Antibody Pair - BSA and Azide free (ab309257)

    Sandwich ELISA of ab309257 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com