Human ALK-1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ALK-1.
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Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
19.53 - 1250 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.
Alternative names
Serine/threonine-protein kinase receptor R3, SKR3, Activin receptor-like kinase 1, TGF-B superfamily receptor type I, ALK-1, TSR-I, ACVRLK1, ALK1, ACVRL1
What's included?
Recommended products
Human ALK-1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ALK-1.
Alternative names
Serine/threonine-protein kinase receptor R3, SKR3, Activin receptor-like kinase 1, TGF-B superfamily receptor type I, ALK-1, TSR-I, ACVRLK1, ALK1, ACVRL1
Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
19.53 - 1250 pg/mL
- Assay Platform
Reagents
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
- Human ALK-1 Antibody Pair - BSA and Azide free (ab305183) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to (quantify Human ALK-1) in sandwich ELISAs and many other pair-based applications.
- The pair can be used in variety of assays and platforms including but not limited to:
- - Sandwich ELISA
- - FRET/TR-FRET/HTR
- - Meso Scale Discovery® (MSD®)
- - Luminex® and bead-based assays
- - AlphaLISA®/AlphaScreen®
- - DELFIA® immunoassays
- - Simoa® and Single Molecule Counting (SMC™) immunoassays
- - Multiplex
- Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
- - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- - Concentration of ~1 mg/ml as measured by the protein A280 method.
- Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
- We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service
- Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
- Please note:
- The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
- The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
- We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
- Antibody properties:
- Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
- Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
- Concentration: ~1 mg/ml
- Storage buffer: 100% PBS
- Form: Liquid
- Isotype: IgG
- Recombinant monoclonal antibodies offer several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
- Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
- Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
- Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
- AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
- Simoa is a registered trademark of Quanterix, Inc.
- SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
ab305183 is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify STING Synaptopodin in sandwich ELISAs and many other pair-based applications.
The pair can be used in variety of assays and platforms including but not limited to:
• Sandwich ELISA
• FRET/TR-FRET/HTRF
• Meso Scale Discovery® (MSD®)
• Bead-based assays
• AlphaLISA®/AlphaScreen®
• DELFIA® immunoassays
• Simoa® and Single Molecule Counting (SMC™) immunoassays
• Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
• Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
• Concentration of ~1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services. Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ALK-1 also known as ACVRL1 or ALK1 protein is a receptor serine/threonine kinase with a molecular mass of approximately 55 kDa. It plays an important role in the TGF-beta superfamily signaling particularly in angiogenesis and blood vessel development. Expressed mainly in endothelial cells ALK-1 mediates cellular responses essential for maintaining vascular stability. The careful balance of its signaling is necessary indicating its importance in physiological conditions.
- Biological function summary
The function of ALK-1 extends to the formation and maintenance of vascular endothelium as part of the larger receptor complex. It interacts with ligands such as BMP9 and BMP10 which regulate endothelial cell proliferation and differentiation. ALK-1's role as part of this receptor complex highlights its involvement in controlling the fine balance between angiogenic and anti-angiogenic processes. Its biological activity is vital for healthy vascular development and homeostasis.
- Pathways
ALK-1 activation is a part of the BMP signaling pathway a branch of the larger TGF-beta pathway. This pathway is significant in various cellular processes including growth and differentiation. ALK-1 interacts with other signalling mediators like SMAD1/5/8 proteins which translocate to the nucleus to affect gene expression. Connecting with these pathways ALK-1 aligns closely with ACVR2A and ENG (Endoglin) which further highlight its role in vascular biology.
- Associated diseases and disorders
ALK-1 mutations or dysregulation leads to conditions such as hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH). These conditions showcase the important role ALK-1 plays in vascular integrity and signaling. ALK-1 is connected to disorders through interactions with proteins such as ENG further highlighting its involvement in vascular-related pathologies. Maintaining precise ALK-1 function is pivotal to preventing these disorders indicating its potential as a therapeutic target or biomarker.
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1 product image
Sandwich ELISA - Human ALK-1 Antibody Pair - BSA and Azide free (ab305183)
Sandwich ELISA of ab305183 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.
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