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AB270346

Human Alpha-synuclein Antibody Pair - BSA and Azide free

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Human Alpha-synuclein Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Alpha-synuclein.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

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Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)
  • sELISA

Lab

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament standard curve.

Background subtracted standard curve using Human Alpha-synuclein Antibody Pair - BSA and Azide free (ab270346) and Recombinant human Alpha-Synuclein protein filament (ab254309) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260052), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260052), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260052), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)
  • sELISA

Supplier Data

Sandwich ELISA - Human Alpha-synuclein Antibody Pair - BSA and Azide free (AB270346)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260052), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

614.54 - 7000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

The Antibody Pair can be used to quantify Human Alpha-synuclein. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab260052), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Product protocols

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SNCA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Assay Kits

AB260052

Human Alpha-synuclein ELISA Kit

elisa-kits

human-alpha-synuclein-elisa-kit-ab260052

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(2 reviews)

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