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Human ATG14L Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ATG14L.

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Images

Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (AB322647), expandable thumbnail
  • Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (AB322647), expandable thumbnail
  • Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (AB322647), expandable thumbnail

Key facts

Assay type

ELISA set

Reactive species

Human

Range

15.63 - 2000 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604, PubMed:37632749). Modulates the hepatic lipid metabolism (By similarity).

Alternative names

What's included?

10 x 96 Tests
Components
Human ATG14L Capture Antibody (unconjugated)
1 x 100 µg
Human ATG14L Detector Antibody (unconjugated)
1 x 100 µg

Human ATG14L Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human ATG14L.

Key facts

Assay type

ELISA set

Reactive species

Human

Range

15.63 - 2000 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human ATG14L Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human ATG14L in sandwich ELISAs and many other pair-based applications.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

The pair can be used in variety of assays and platforms including but not limited to:


  • Sandwich ELISA

  • FRET/TR-FRET/HTRF

  • Meso Scale Discovery® (MSD®)

  • Bead-based assays

  • AlphaLISA®/AlphaScreen®

  • DELFIA® immunoassays

  • Simoa® and Single Molecule Counting (SMC™) immunoassays

  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:


  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647), expandable thumbnail

    Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647)

    Sandwich ELISA of ab322647 with the capture antibody Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) ab322648 dilution at 2 ug/mL and detector antibody Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) ab322649 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647), expandable thumbnail

    Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647)

    Interpolated concentrations of native ATG14L in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and ATG14 (ATG14L) knockout HeLa cell based on 250 ug/mL extract loads. The concentrations of ATG14L were measured in duplicate and interpolated from the ATG14L standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ATG14L concentration was determined to be 397.4 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line Human wild-type HeLa cell line ab255448) and 50.4 pg/mL in ATG14 (ATG14L) knockout HeLa extract (Human ATG14 (ATG14L) knockout HeLa cell line Human ATG14 (ATG14L) knockout HeLa cell line ab264684).

  • Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647), expandable thumbnail

    Sandwich ELISA - Human ATG14L Antibody Pair - BSA and Azide free (ab322647)

    Interpolated concentration of native ATG14L was measured at different sample concentrations. Undiluted samples are as follows: HeLa cell extract (250 ug/mL) and HCT116 cell extract (400 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com