JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB290702

Human c-Myc Antibody Pair - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Human c-Myc Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human c-Myc.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

2 Images
Sandwich ELISA - Human c-Myc Antibody Pair - BSA and Azide free (AB290702)
  • sELISA

Supplier Data

Sandwich ELISA - Human c-Myc Antibody Pair - BSA and Azide free (AB290702)

Sandwich ELISA of ab290702 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

Sandwich ELISA - Human c-Myc Antibody Pair - BSA and Azide free (AB290702)
  • sELISA

Lab

Sandwich ELISA - Human c-Myc Antibody Pair - BSA and Azide free (AB290702)

Interpolated concentrations of native c-Myc in human control wild type HEK293T cell and MYC knockout HEK293T cell based on 1000 μg/mL extract loads. The concentrations of c-Myc were measured in duplicate and interpolated from the c-Myc standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean c-Myc concentration was determined to be 4509.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in MYC knockout HEK293T extract (Human MYC knockout HEK293T cell line ab256500).

Key facts

Reacts with

Human

Range

62.5 - 8000 pg/mL

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human c-Myc Antibody Pair - BSA and Azide free (ab290702) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human c-myc in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab290702-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human c-Myc Capture Antibody (unconjugated)", "number":"AB290702-CMP02", "productcode":"AB290674" }, { "size":"1 x 100 µg", "name":"Human c-Myc Detector Antibody (unconjugated)", "number":"AB290702-CMP01", "productcode":"AB290675" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
style
left-column

Product protocols

style
left-column

Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC
style
left-column
style
left-column
style
right-column

Complementary Products

Sample Prep & Detection Kits

AB201796

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®

Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)

  • 3
  • 1
Primary Antibodies

AB109307

Anti-Axin 2 antibody [EPR2005(2)]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (AB109307)

  • 4
  • 5
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 55
Proteins & Peptides

AB169901

Recombinant human c-Myc protein (Active)

  • 5
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com