Human C3a anaphylatoxin Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human C3a anaphylatoxin.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 7.81 - 500 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512).
Alternative names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
What's included?
Recommended products
Human C3a anaphylatoxin Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human C3a anaphylatoxin.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 7.81 - 500 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C3a anaphylatoxin also known simply as C3a functions as a small protein fragment within the complement system. It forms during the cleavage of complement component 3 (C3) and has a molecular mass of approximately 9 kDa. C3a is mainly expressed on leukocytes endothelial cells and smooth muscle cells. It acts as a potent mediator of inflammation by binding to the C3a receptor (C3aR) found on the surface of various immune cells like mast cells and basophils.
- Biological function summary
C3a interacts extensively with cells involved in immune responses. It serves as an inflammatory mediator promoting chemotaxis mast cell degranulation and increased vascular permeability. C3a is not part of a large complex but acts independently to drive immune responses through its receptors. It also plays a role in the process of immune regulation and tissue repair by attracting immune cells to sites of infection or damage.
- Pathways
C3a participates in both the complement and coagulation cascades. In the complement pathway it functions alongside other components like C5a and C5b-9 enhancing the local immune responses and pathogen clearance. The coagulation pathway also involves interactions with various other proteins affecting cell signaling and inflammation bridging innate and adaptive immunity.
- Associated diseases and disorders
C3a contributes to conditions like asthma and autoimmune disorders. In asthma it promotes airway inflammation and hyperresponsiveness by acting on immune cells and tissues. In systemic lupus erythematosus (SLE) C3a levels correlate with inflammation and tissue damage involving proteins in immune complex deposition. Understanding C3a's activity helps in developing therapeutic strategies targeting inflammatory diseases.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
Sandwich ELISA - Human C3a anaphylatoxin Antibody Pair - BSA and Azide free (ab316993)
Sandwich ELISA of ab316993 with the capture antibody Anti-C3a anaphylatoxin antibody [EPR26616-82] - BSA and Azide free (Capture) ab316994 dilution at 2 ug/mL and detector antibody Anti-C3a anaphylatoxin antibody [EPR26616-22] - BSA and Azide free (Detector) ab316995 dilution at 0.5 ug/mL.
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