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Human CBL Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CBL.

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Images

Key facts

Assay type

ELISA set

Reactive species

Human

Range

0.08 - 10 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:17094949). Ubiquitinates SPRY2 (PubMed:17094949, PubMed:17974561). Ubiquitinates EGFR (PubMed:17974561). Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).

Alternative names

What's included?

10 x 96 Tests
Components
Human CBL Capture Antibody (unconjugated)
1 x 100 µg
Human CBL Detector Antibody (unconjugated)
1 x 100 µg

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Human CBL Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CBL.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Human

Range

0.08 - 10 ng/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Supplementary info

Biological function summary

The CBL protein acts as a negative regulator for several signaling pathways. It integrates into signaling complexes and modulates the activation of pathways by interacting with other proteins. CBL does not act alone; it commonly forms complexes with proteins such as Grb2 and p85 influencing downstream signaling influence in pathways. This regulation prevents excessive activation of signals that can lead to cellular proliferation or differentiation abnormally.

Activity summary

The CBL protein also known as C-cbl or Casitas B-lineage lymphoma proto-oncogene serves as an E3 ubiquitin-protein ligase. This protein with a molecular mass of approximately 120-130 kDa plays a critical role in the regulation of receptor tyrosine kinases. It facilitates the polyubiquitination of activated receptor proteins targeting them for degradation in the proteasome. CBL is predominantly expressed in various tissues including the hematopoietic system. It is a significant component in the control of cell signaling and helps maintain cellular homeostasis.

Pathways

CBL integrates into the ubiquitin-proteasome system and the Ras signaling pathway. Within these pathways CBL works to modulate cell surface receptors ensuring that signaling threshold is not surpassed. The importance of CBL in the Ras signaling pathway is highlighted by its interactions with proteins like Ras-GAP and SHP2. Through these interactions CBL controls the rate of signal transduction affecting cellular responses such as growth survival and motility.

Associated diseases and disorders

CBL involvement has been linked to several forms of cancer and immune deficiencies. Mutations or dysregulation of the CBL gene can lead to myelodysplastic syndromes and acute myeloid leukemia. In these conditions the interaction between CBL and other proteins such as JAK2 becomes dysregulated driving abnormal cell growth and survival. Furthermore CBL’s failure to properly degrade tyrosine kinase receptors can contribute to enhanced signaling fostering oncogenic processes.

Product promise

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4 product images

  • Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114), expandable thumbnail

    Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114)

    Sandwich ELISA of ab322114 with the capture antibody ab322115 dilution at 2 ug/mL and detector antibody ab322116 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114), expandable thumbnail

    Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114)

    Interpolated concentration of native CBL was measured at different sample concentrations. Undiluted sample is as follows: HEK293T cell extract (25 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in antibody diluent CP (ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114), expandable thumbnail

    Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114)

    Interpolated concentration of native CBL was measured at different sample concentrations. Undiluted samples are as follows: human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP (ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114), expandable thumbnail

    Sandwich ELISA - Human CBL Antibody Pair - BSA and Azide free (ab322114)

    Interpolated concentrations of native CBL in human control wild type HEK293T (human embryonic kidney epithelial cell) and CBL knockout HEK293T cell based on 100 ug/mL extract loads. The concentrations of CBL were measured in duplicate and interpolated from the CBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CBL concentration was determined to be 8939 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in CBL knockout HEK293T extract (Human CBL knockout HEK293T cell line ab267245).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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