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Human CD47 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CD47.

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Key facts

Assay type

ELISA set

Reactive species

Human

Range

125 - 8000 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Alternative names

What's included?

10 x 96 Tests
Components
Human CD47 Capture Antibody (unconjugated)
1 x 100 µg
Human CD47 Detector Antibody (unconjugated)
1 x 100 µg

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Human CD47 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CD47.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Human

Range

125 - 8000 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CD47 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human CD47 in sandwich ELISAs and many other pair-based applications.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:


  • Sandwich ELISA

  • FRET/TR-FRET/HTRF

  • Meso Scale Discovery® (MSD®)

  • Bead-based assays

  • AlphaLISA®/AlphaScreen®

  • DELFIA® immunoassays

  • Simoa® and Single Molecule Counting (SMC™) immunoassays

  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:


  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com