Skip to main content

Human CFHR3 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CFHR3.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human CFHR3 Antibody Pair - BSA and Azide free (AB305094), expandable thumbnail

Key facts

Assay type

ELISA set

Reactive species

Human

Range

7.8 - 1000 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Might be involved in complement regulation.

Alternative names

Complement factor H-related protein 3, FHR-3, DOWN16, H factor-like protein 3, FHR3, CFHR3, CFHL3

What's included?

10 x 96 Tests
Components
ab305160
Human CFHR3 Capture Antibody (unconjugated)
1 x 100 µg
ab305161
Human CFHR3 Detector Antibody (unconjugated)
1 x 100 µg

Recommended products

Human CFHR3 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CFHR3.

Alternative names

Complement factor H-related protein 3, FHR-3, DOWN16, H factor-like protein 3, FHR3, CFHR3, CFHL3

Key facts

Assay type

ELISA set

Reactive species

Human

Range

7.8 - 1000 pg/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

  • Human CFHR3 (Complement factor H-related protein 3) Antibody Pair - BSA and Azide free (ab305094) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human CFHR3 in sandwich ELISAs and many other pair-based applications.
  • The pair can be used in variety of assays and platforms including but not limited to:
  • - Sandwich ELISA
  • - FRET/TR-FRET/HTR
  • - Meso Scale Discovery® (MSD®)
  • - Luminex® and bead-based assays
  • - AlphaLISA®/AlphaScreen®
  • - DELFIA® immunoassays
  • - Simoa® and Single Molecule Counting (SMC™) immunoassays
  • - Multiplex

  • Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
  • - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • - Concentration of ~1 mg/ml as measured by the protein A280 method.

  • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
  • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service

  • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

  • Please note:
  • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
  • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
  • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

  • Antibody properties:
  • Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
  • Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
  • Concentration: ~1 mg/ml
  • Storage buffer: 100% PBS
  • Form: Liquid
  • Isotype: IgG

  • Recombinant monoclonal antibodies offer several advantages including:
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
  • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
  • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
  • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
  • Simoa is a registered trademark of Quanterix, Inc.
  • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Complement factor H-related protein 3 (CFHR3) also known as FHR-3 plays a role in modulating the complement system. This protein has a mass of approximately 42 kDa. CFHR3 is expressed in tissues like the liver and can be found circulating in the plasma. It belongs to the factor H protein family which participates in maintaining immune homeostasis by regulating complement activation.

Biological function summary

CFHR3 acts as an antagonist to complement factor H by competing for binding sites on target surfaces and complement components. It does not function as a standalone protein but interacts closely within a family that includes CFH and other complement factor H-related proteins. CFHR3 can potentially form complexes with similar proteins to help finely tune the activity of the complement system to prevent excessive immune response.

Pathways

CFHR3 is a part of the complement cascade system specifically the alternative pathway. It contributes to the negative regulation of this pathway which is important for preventing overactivation that could harm host tissues. The protein influences the binding of complement proteins like C3b an essential component in the complement cascade and interacts with other factor H-related proteins such as CFHR1 to modulate pathway activities.

Associated diseases and disorders

CFHR3's regulatory role connects it to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS mutations or deletions in CFHR3 sometimes in conjunction with CFHR1 can lead to uncontrolled complement activation contributing to disease progression. Similarly variations in CFHR3's sequence can affect AMD risk often in association with other complement regulatory proteins including CFH indicating its significant position in disease susceptibility through its regulatory function within the immune system.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human CFHR3 Antibody Pair - BSA and Azide free (ab305094), expandable thumbnail

    Sandwich ELISA - Human CFHR3 Antibody Pair - BSA and Azide free (ab305094)

    Human CFHR3 Antibody Pair.

    Sandwich ELISA of ab305094 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com