Human DCBLD2/ESDN Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human DCBLD2/ESDN.
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Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
31.25 - 2000 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Endothelial and smooth muscle cell-derived neuropilin-like protein, DCBLD2, ESDN, CLCP1
What's included?
Human DCBLD2/ESDN Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human DCBLD2/ESDN.
Alternative names
Endothelial and smooth muscle cell-derived neuropilin-like protein, DCBLD2, ESDN, CLCP1
Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
31.25 - 2000 pg/mL
- Assay Platform
Reagents
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human DCBLD2/ESDN Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human DCBLD2/ESDN in sandwich ELISAs and many other pair-based applications.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human DCBLD2/ESDN Antibody Pair - BSA and Azide free (ab322472)
Sandwich ELISA of ab322472 with the capture antibody Anti-DCBLD2/ESDN antibody [EPR30139-524] - BSA and Azide free (Capture) ab322473 dilution at 2 ug/mL and detector antibody Anti-DCBLD2/ESDN antibody [EPR30139-520] - BSA and Azide free (Detector) ab322474 dilution at 0.5 ug/mL.
Sandwich ELISA - Human DCBLD2/ESDN Antibody Pair - BSA and Azide free (ab322472)
Interpolated concentration of native DCBLD2/ESDN was measured at different sample concentrations. Undiluted sample is as follows: A375 cell extract (10 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Sandwich ELISA - Human DCBLD2/ESDN Antibody Pair - BSA and Azide free (ab322472)
Interpolated concentration of native DCBLD2/ESDN was measured at different sample concentrations. Undiluted samples are as follows: human serum 5% and human plasma (EDTA) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
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Product protocols
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