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AB253341

Human DPT Antibody Pair - BSA and Azide free (TRAMP)

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Human DPT Antibody Pair - BSA and Azide free (TRAMP) is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human DPT (TRAMP).

View Alternative Names

Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

1 Images
Sandwich ELISA - Human DPT Antibody Pair - BSA and Azide free (TRAMP) (AB253341)
  • sELISA

Supplier Data

Sandwich ELISA - Human DPT Antibody Pair - BSA and Azide free (TRAMP) (AB253341)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272206), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

18.75 - 1200 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

The Antibody Pair can be used to quantify Human DPT (TRAMP). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab272206), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.
Biological function summary

Dermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.

Pathways

Dermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.

Dermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.
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Product protocols

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Target data

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).
See full target information Dermatopontin
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