Human FOLR3 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human FOLR3.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 15.6 - 2000 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate.
Alternative names
Folate receptor gamma, FR-gamma, Folate receptor 3, FOLR3
What's included?
Recommended products
Human FOLR3 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human FOLR3.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 15.6 - 2000 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human FOLR3 Antibody Pair - BSA and Azide free (ab303703) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to (quantify human Aquaporin 4) in sandwich ELISAs and many other pair-based application.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOLR3 also known as folate receptor gamma is a member of the folate receptor family responsible for binding and transporting folate across cell membranes. This protein has a molecular mass of about 32 kDa. FOLR3 is primarily found in certain tissues including the placenta and liver and it exhibits limited expression in several epithelial tissues. Folate receptors including FOLR3 function by binding folates and delivering them into cells via endocytosis a process essential for the maintenance of regular cellular activity and function.
- Biological function summary
Folate receptor gamma plays a significant role in the transport and uptake of folate an important vitamin necessary for DNA synthesis and methylation. The receptor assists in various cellular processes including nucleotide biosynthesis. FOLR3 works as part of a larger complex of folate receptors which ensure cells have adequate folate levels for normal metabolic functions. By doing so it helps facilitate normal cell growth and division critical in rapidly dividing cells.
- Pathways
FOLR3 is notably involved in the folate metabolism pathway contributing to DNA synthesis and repair processes. This pathway connects with the one-carbon metabolism pathway where folate is an essential donor of carbons necessary for methylation reactions. In these pathways FOLR3 interacts with other folate receptors like FOLR1 and FOLR2 to maintain efficient folate transport and cellular homeostasis. Ensuring proper folate availability supports homocysteine metabolism which is another critical aspect of these pathways.
- Associated diseases and disorders
FOLR3 relates notably to conditions such as cancer and inflammatory disorders. Cancer cells often exhibit elevated folate receptor expression including FOLR3 due to their increased requirement for folate in rapid cell proliferation. Inflammatory disorders also see changes in folate receptor expression which might affect immune cell functions and inflammation regulation. In these contexts FOLR3 interacts with tumor-associated proteins and immune modulators highlighting its potential as a therapeutic target in medical research and treatment development.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human FOLR3 Antibody Pair - BSA and Azide free (ab303703)
Human FLOR3 antibody pair.
Sandwich ELISA of ab303703 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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