Human Gastric Mucin/MUC-6 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Gastric Mucin/MUC-6.
ELISA set
Human
39 - 2500 pg/mL
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
Mucin-6, MUC-6, Gastric mucin-6, MUC6
Human Gastric Mucin/MUC-6 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Gastric Mucin/MUC-6.
ELISA set
Human
39 - 2500 pg/mL
Reagents
Blue Ice
+4°C
+4°C
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
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This supplementary information is collated from multiple sources and compiled automatically.
MUC-6 also called gastric mucin is a large glycoprotein with a molecular mass around 500-1000 kDa. Cells in the stomach express MUC-6 where it plays a critical mechanical role in forming protective mucous barriers against harsh gastric environments. The glycoprotein forms viscous gels that coat the gastric mucosa creating a protective layer. These layers prevent autodigestion by gastric acid and pepsin safeguarding the underlying epithelium from damage.
MUC-6 contributes to the defense of gastric tissues through its complex structure. The protein belongs to the mucin family characterized by their high carbohydrate content. MUC-6 interacts with other secreted mucins like MUC-5AC to form a protective gel. It acts as an important component in the maintenance of the gastric mucosal barrier ensuring the separation of stomach contents and epithelial surface. This gel traps pathogens and particles reducing their ability to cause infection or irritation.
MUC-6 plays a role in mucosal immunity and epithelial signaling pathways. In the gastric mucosa pathway it links with proteins such as CLCA1 which influences mucin secretion and function. Another linked pathway is the oxidative stress pathway where MUC-6 partners with antioxidant enzymes to shield the stomach lining from oxidative damage. These pathways showcase how MUC-6 integrates a mechanical and biological defense system within the digestive tract.
Alterations in MUC-6 expression levels can influence conditions like gastric cancer and peptic ulcer disease. In gastric cancer abnormal methylation of the MUC-6 gene can contribute to tumorigenesis and tumor progression. In peptic ulcer disease deficient mucin levels particularly MUC-6 compromise the mucosal barrier function. Connections also exist with proteins like gastrin which elevate acid production and burden the mucosal defense emphasizing the relevance of MUC-6 in these gastric conditions.
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Sandwich ELISA of ab320665 with the capture antibody Anti-Gastric Mucin/MUC-6 antibody [EPR27887-272] - BSA and Azide free (Capture) ab320666 dilution at 2 ug/mL and detector antibody Anti-Gastric Mucin/MUC-6 antibody [EPR27887-260] - BSA and Azide free (Detector) ab320667 dilution at 0.5 ug/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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