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AB222279

Human GFAP Matched Antibody Pair Kit

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(5 Publications)

Human GFAP Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human GFAP by sandwich ELISA method.

View Alternative Names

Glial fibrillary acidic protein, GFAP

1 Images
Sandwich ELISA - Human GFAP Matched Antibody Pair Kit (AB222279)
  • sELISA

Supplier Data

Sandwich ELISA - Human GFAP Matched Antibody Pair Kit (AB222279)

Human GFAP standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Human

Assay type

ELISA set

Sensitivity

= 3.47 pg/mL

Range

15.6 - 1000 pg/mL

Assay Platform

Reagents

Reactivity data

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Product details

Human GFAP Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human GFAP. Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905). For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab223867. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Mikrochimica acta 190:493 PubMed38032374

2023

Palladium nanoclusters as a label to determine GFAP in human serum from donors with stroke by bimodal detection: inductively coupled plasma-mass spectrometry and linear sweep voltammetry.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro Rodríguez-Penedo,Estefanía Costa-Rama,Beatriz Fernández,Carmen García-Cabo,Lorena Benavente,Sergio Calleja,M Teresa Fernández-Abedul,Rosario Pereiro

Scientific reports 13:12826 PubMed37550376

2023

Acute brain slice elastic modulus decreases over time.

Applications

Unspecified application

Species

Unspecified reactive species

John Exton,Jonathan M G Higgins,Jinju Chen

ACS sensors 7:253-262 PubMed34908400

2021

Detection of Glial Fibrillary Acidic Protein in Patient Plasma Using On-Chip Graphene Field-Effect Biosensors, in Comparison with ELISA and Single-Molecule Array.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhou Xu,Sami Ramadan,Oluwatomi E Akingbade,Yuanzhou Zhang,Sarah Alodan,Neil Graham,Karl A Zimmerman,Elias Torres,Amanda Heslegrave,Peter K Petrov,Henrik Zetterberg,David J Sharp,Norbert Klein,Bing Li

Biosensors 11: PubMed34562910

2021

A Variable Height Microfluidic Device for Multiplexed Immunoassay Analysis of Traumatic Brain Injury Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Alyse D Krausz,Frederick K Korley,Mark A Burns

Diagnostics (Basel, Switzerland) 11: PubMed34206615

2021

A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo Gaude,Barbara Nogueira,Marcos Ladreda Mochales,Sheila Graham,Sarah Smith,Lisa Shaw,Sara Graziadio,Gonzalo Ladreda Mochales,Philip Sloan,Joshua D Bernstock,Shashank Shekhar,Toby I Gropen,Christopher I Price
View all publications
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Product promise

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