Human GFAP Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human GFAP by sandwich ELISA method.
Application | Reactivity | Dilution info | Notes |
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Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP
Human GFAP Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human GFAP by sandwich ELISA method.
Human GFAP Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human GFAP. Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ELISA Accessory Pack ab210905). For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit Human GFAP ELISA Kit ab223867. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Human GFAP standard curve.
Standard calibration curve. Background subtracted values are graphed.
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