Human IgG4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human IgG4.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 9.8 - 2500 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
What's included?
Recommended products
Human IgG4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human IgG4.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 9.8 - 2500 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IgG4 Antibody Pair - BSA and Azide free (ab309267) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human IgG4 in sandwich ELISAs and many other pair-based applications.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human IgG4 Antibody Pair - BSA and Azide free (ab309267)
Sandwich ELISA of ab309267 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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