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Human IL-18 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IL-18 by sandwich ELISA method.

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Sandwich ELISA - Human IL-18 Matched Antibody Pair Kit (AB218185), expandable thumbnail

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Key facts

Detection method
Colorimetric
Assay type
ELISA set
Reactive species
Human
Range
31.25 - 2000 pg/mL
Sensitivity
= 13.6 pg/mL

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed:14528293, PubMed:25500532, PubMed:37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed:10653850). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744).

Alternative names

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

What's included?

5 x 96 Tests
Components
Human IL-18 Capture Antibody
1 x 50 µg
Human IL-18 Detector Antibody
1 x 12.5 µg
Human IL-18 Lyophilized Protein
1 x 1 Vial

Recommended products

Human IL-18 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IL-18 by sandwich ELISA method.

Key facts

Detection method
Colorimetric
Assay type
ELISA set
Reactive species
Human
Range
31.25 - 2000 pg/mL
Assay Platform
Reagents
Sensitivity
= 13.6 pg/mL

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human IL-18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-18.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ELISA Accessory Pack ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit Human IL-18 ELISA Kit ab215539. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

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1 product image

  • Sandwich ELISA - Human IL-18 Matched Antibody Pair Kit (ab218185), expandable thumbnail

    Sandwich ELISA - Human IL-18 Matched Antibody Pair Kit (ab218185)

    Human IL-18 standard curve.

    Standard calibration curve. Background subtracted values are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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