JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB217608

Human IL-1beta Matched Antibody Pair Kit

Be the first to review this product! Submit a review

|

(11 Publications)

Human IL-1beta Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IL-1beta by sandwich ELISA method.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

1 Images
ELISA - Human IL-1beta Matched Antibody Pair Kit (AB217608)
  • ELISA

Supplier Data

ELISA - Human IL-1beta Matched Antibody Pair Kit (AB217608)

Human IL-1beta standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Human

Assay type

ELISA set

Sensitivity

= 12.9 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Reagents

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human IL-1beta Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-1beta.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214025. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab217608-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Human IL-1 beta Lyophilized Protein", "number":"AB217608-CMP01", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Human IL-1 beta Detector Antibody", "number":"AB217608-CMP03", "productcode":"" }, { "size":"1 x 50 µg", "name":"Human IL-1 beta Capture Antibody", "number":"AB217608-CMP02", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).
See full target information IL1B

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40372612

2025

LncRNA SNHG7/miR-181b-5p/TLR4 Activates Inflammation And Promotes Pyroptosis Through NF-κB Signaling in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Sheng-Jiang Xue,Feng Yang,Meng Xiao,Yong-Bo Tang,Ying Wu

Cell death & disease 15:400 PubMed38849380

2024

Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxiang Wu,Pin Wang,Xinyu Xie,Xiaoqi Yang,Shuangmei Tang,Jiping Zhao,Tian Liu,Junfei Wang,Jintao Zhang,Tongliang Xia,Xin Feng

Medicine 102:e32384 PubMed36820569

2023

Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Yang,Youjiang Qiu

Cytokine 153:155868 PubMed35358903

2022

Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo Brambila

BMC pharmacology & toxicology 22:62 PubMed34686215

2021

Famotidine promotes inflammation by triggering cell pyroptosis in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Huang,Pingsheng Fan,Miao Liu,Chengtao Weng,Gaofei Fan,Tengyue Zhang,Xiaohong Duan,Yang Wu,Lili Tang,Guohong Yang,Yabei Liu

Journal of cellular and molecular medicine 25:9983-9994 PubMed34664412

2021

Dexmedetomidine alleviates hepatic ischaemia-reperfusion injury via the PI3K/AKT/Nrf2-NLRP3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wu,Gaolin Qiu,Hainie Zhang,Leilei Zhu,Gao Cheng,Yiqiao Wang,Yuanhai Li,Weiwei Wu

Journal of inflammation research 14:4069-4077 PubMed34456582

2021

The Relationship Between Circulating Bone Morphogenetic Protein-4 and Inflammation Cytokines in Patients Undergoing Thoracic Surgery: A Prospective Randomized Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhao,Jitao Zhang,Wenjuan Zhang,Ruping Dai,Junmei Xu,Zhijian Li,Lin Yang

BMC pharmacology & toxicology 22:44 PubMed34266494

2021

Rabeprazole inhibits inflammatory reaction by inhibition of cell pyroptosis in gastric epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xie,Long Fan,Liya Xiong,Peiyu Chen,Hongli Wang,Huan Chen,Junhong Zhao,Zhaohui Xu,Lanlan Geng,Wanfu Xu,Sitang Gong

Oxidative medicine and cellular longevity 2021:2580619 PubMed34122720

2021

MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury the NOTCH2/Abcb1a Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Changzhao Gao,Fei Yin,Ran Li,Qing Ruan,Chunyang Meng,Kunchi Zhao,Qingsan Zhu

Molecular medicine reports 23: PubMed33495843

2021

IL-32 exacerbates adenoid hypertrophy via activating NLRP3-mediated cell pyroptosis, which promotes inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Junmei Zhang,Xuyuan Sun,Lingling Zhong,Bei Shen
View all publications
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com