Human IL-5 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IL-5 by sandwich ELISA method.
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Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 3.9 - 250 pg/mL
- Sensitivity
- = 0.45 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
Alternative names
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
What's included?
Recommended products
Human IL-5 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IL-5 by sandwich ELISA method.
Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 3.9 - 250 pg/mL
- Assay Platform
- Reagents
- Sensitivity
- = 0.45 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human IL-5 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-5.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ELISA Accessory Pack ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit Human IL-5 ELISA Kit ab215536. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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1 product image
ELISA - Human IL-5 Matched Antibody Pair Kit (ab216795)
Human IL-5 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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