Human IRAK4 Matched Antibody Pair Kit

Product details

Human IRAK4 Matched Antibody Pair Kit is an ELISA development kit to quantify Human IRAK4 by sandwich ELISA method with a sensitivity of 46.8 pg/mL.

How the Assay Works

A capture antibody specific of the antigen to be measured is coated on a 96-well plate. Samples are added to the wells followed by a biotin-labelled antibody. After elimination of the unbound antigen and detection antibody, a strepatavidin-HRP solution is added to the wells, followed by a TMB substrate. HRP catalyzes the oxidation of TMB in the presence of hydrogen peroxide, producing a blue-colored product. After the reaction is stopped, the solution turns yellow. The intensity of the yellow color, read at 450nm is directly proportional to the amount of antigen present in the sample.

Assay specificity

The Matched Antibody Pair Kit can be used to quantify native and recombinant human IRAK4.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.

protocol summary

1. Coat the 96-well plate with the capture antibody overnight at 4C or 2 hours at room temperature on a plate shaker
2. Wash and block to reduce non specific binfing.
3. Add standards and samples to appropiate wells and incubate at room temperature.
4. Wash
5. Add the biotin-labelled detector antibody to all wells and incubate at room temperature
6. Wash
7. Add the HRP-Streptavidin solution and incubate at room temperature
8. Wash
9. Add the TMB substrate solution and incubate
10. Add stop solution and read at 450 nm

Complete your experiment with:

Matched Antibody Pair Kit ELISA Accessory Pack (10 x 96-well plates) (ab210905): contains 10 96-well plates, coating and blocking buffers, wash buffer, TMB substrate, stop solution and streptavidin-HRP solution.