Human KMT2B / MLL4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human KMT2B / MLL4.
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Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
78.13 - 5000 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Histone methyltransferase that methylates 'Lys-4' of histone H3 (PubMed:17707229). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:17707229). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed:17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).
Alternative names
Histone-lysine N-methyltransferase 2B, Lysine N-methyltransferase 2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7, MLL4, WBP7, TRX2, KMT2B, MLL2, HRX2, KIAA0304
What's included?
Recommended products
Human KMT2B / MLL4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human KMT2B / MLL4.
Alternative names
Histone-lysine N-methyltransferase 2B, Lysine N-methyltransferase 2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7, MLL4, WBP7, TRX2, KMT2B, MLL2, HRX2, KIAA0304
Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
78.13 - 5000 pg/mL
- Assay Platform
Reagents
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT2B also known as MLL4 is a protein complex that functions as a histone methyltransferase. It specifically catalyzes the methylation of histone H3 on lysine 4 (H3K4) a modification associated with transcriptional activation. KMT2B has a molecular mass of approximately 296 kDa and is widely expressed in human tissues with notable expression in the brain heart and skeletal muscle. This protein is part of the COMPASS-like complexes which play significant roles in chromatin remodeling.
- Biological function summary
KMT2B contributes to regulating gene expression. As part of the larger COMPASS-like complex it cooperates with other regulatory proteins to modify chromatin structure facilitating easier access of transcriptional machinery to DNA. This action influences the activation of genes important for cell differentiation and development. KMT2B interacts with several cofactors including WRAD components (WDR5 RBBP5 ASH2L and DPY30) enhancing its enzymatic activity.
- Pathways
KMT2B functions prominently in signaling mechanisms like the Wnt and MAPK pathways. These pathways are integral to cell growth and differentiation. The protein intricately connects with other pathways’ proteins including beta-catenin in the Wnt pathway and ERK1/2 in the MAPK pathway. These interactions highlight KMT2B's role in regulating cellular functions and development.
- Associated diseases and disorders
KMT2B has associations with neurodevelopmental disorders and specific forms of cancer. Mutations in KMT2B can lead to dystonia a movement disorder characterized by involuntary muscle contractions. In cancer alterations affect pathways involving oncogenic factors such as MYC highlighting the critical connections between KMT2B and cellular growth regulation. Understanding these links provides insights into potential therapeutic targets for related conditions.
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Sandwich ELISA - Human KMT2B / MLL4 Antibody Pair - BSA and Azide free (ab317052)
Sandwich ELISA of ab317052 with the capture antibody Anti-KMT2B / MLL4 antibody [EPR27970-43] - BSA and Azide free (Capture) ab317053 dilution at 2 ug/mL and detector antibody Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR27970-83] - BSA and Azide free (Detector) ab317054 dilution at 0.5 ug/mL.
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