Human LC3A Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human LC3A.
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- Bioengineered 12:12227-122352021Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhonghai Xie,Hongwei Li,Jin ZangPubMed 34783291
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538).
Alternative names
Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/MAP1B LC3 A, MAP1LC3A
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Human LC3A Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human LC3A.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
The Antibody Pair can be used to quantify Human LC3A. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (Human LC3A ELISA Kit ab239432), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein LC3A also known as Microtubule-associated proteins 1A/1B light chain 3A plays an important role in the autophagy process. It exists in two forms LC3-I and LC3-II with the latter being lipidated and integrated into autophagosomal membranes. LC3A has a molecular mass of approximately 17 kDa. Researchers observe its expression in various tissues but most highly in muscle liver and brain. It is often studied along with LC3B due to their similar roles in autophagy.
- Biological function summary
LC3A participates in the formation of autophagosomes which are double-membraned vesicles that capture cellular waste for degradation. LC3A's conversion from LC3-I to LC3-II on the autophagosomal membrane signals the initiation of autophagy. It associates with proteins in the Atg family forming complexes that regulate the maturation of autophagosomes. The LC3 family including LC3A and LC3B acts as important components in the cell's ability to maintain homeostasis through autophagic activity.
- Pathways
LC3A plays a vital part in the autophagy pathway and is also linked to the mTOR signaling pathway. Autophagy is a process that helps with the recycling of cellular components and mTOR signaling inhibits autophagy when nutrients are abundant. LC3A's interactions with other proteins such as p62 and ULK1 are essential in modulating these processes and ensuring cell survival under stress conditions. LC3A and LC3B's roles are integral in coordinating the cellular response to nutrient status and stress highlighting their importance in cellular regulation.
- Associated diseases and disorders
LC3A has significant connections to cancer and neurodegenerative diseases. Altered autophagic activity where LC3A takes part appears in various forms of cancer indicating its role in tumor progression and apoptosis regulation. Additionally abnormal LC3A function has implications in disorders such as Alzheimer's disease where autophagic dysfunction leads to neurodegeneration. In these contexts LC3A's relationship with proteins like p62 which accumulate in neurodegenerative diseases provides insight into pathological mechanisms and potential therapeutic targets.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Sandwich ELISA - Human LC3A Antibody Pair - BSA and Azide free (ab244175)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human LC3A ELISA Kit ab239432), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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