JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314569

Human LTBR Antibody Pair - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Human LTBR Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human LTBR.

View Alternative Names

D12S370, TNFCR, TNFR3, TNFRSF3, LTBR, Tumor necrosis factor receptor superfamily member 3, Lymphotoxin-beta receptor, Tumor necrosis factor C receptor, Tumor necrosis factor receptor 2-related protein, Tumor necrosis factor receptor type III, TNF-RIII, TNFR-III

1 Images
Sandwich ELISA - Human LTBR Antibody Pair - BSA and Azide free (AB314569)
  • sELISA

Supplier Data

Sandwich ELISA - Human LTBR Antibody Pair - BSA and Azide free (AB314569)

Sandwich ELISA of ab314569 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

1.95 - 250 pg/mL

Assay Platform

Reagents

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab314569-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human LTBR Detector Antibody (unconjugated)", "number":"AB314569-CMP02", "productcode":"AB314571" }, { "size":"1 x 100 µg", "name":"Human LTBR Capture Antibody (unconjugated)", "number":"AB314569-CMP01", "productcode":"AB314570" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LTBR (lymphotoxin beta receptor) also known as the TNFRSF3 is a significant protein that functions as a receptor for lymphotoxin and other related cytokines. The protein has a mass of around 62 kDa and is expressed in various tissues including lymphoid tissues and the epithelial cells of several organs. LTBR is present as a membrane-bound protein and helps mediate signaling that is important for cellular communication and function. Key interactions with ligands such as LTA and LIGHT activate signaling pathways which play roles in immune response.
Biological function summary

LTBR coordinates multiple cellular processes especially in immune regulation and homeostasis. It forms a complex with cytokines that binds to its extracellular domain triggering downstream signaling cascades. These cascades lead to activation of nuclear factor kappa B (NF-kB) which controls gene transcription and promotes survival and differentiation of immune cells. The capability of LTBR to interact with other TNF receptors expands its influence in lymphoid organogenesis and maintenance especially in maintaining the integrity of the lymphoid system.

Pathways

LTBR significantly participates in both the NF-kB and the non-canonical NF-kB pathways. These pathways are essential for regulating immune response and inflammation. Within these pathways LTBR interacts with other proteins such as TRAF3 and TRAF2 to mediate signaling processes. This arrangement allows for modulation of immune responses and is critical for recognizing and eliminating pathogens. Additionally the involvement of LTBR in immune system pathways aligns it with the functions of closely related proteins like TNF receptor superfamily members.

LTBR has been implicated in autoimmune diseases and cancer. In autoimmunity aberrant signaling through LTBR may lead to inappropriate immune responses and inflammation as seen in disorders like systemic lupus erythematosus. Moreover in oncology abnormalities in LTBR signaling can influence tumor microenvironments and support tumor cell survival. Throughout these disease contexts LTBR often interacts with other risk-associated proteins such as NFKB1 highlighting its integration in complex pathological processes.
style
left-column

Product protocols

style
left-column

Target data

Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT (PubMed : 24248355). Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)) (PubMed : 24248355). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
See full target information LTBR
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB320846

Anti-LTBR antibody [RM2069] - BSA and Azide free

Recombinant|RabMAb

Flow Cytometry - Anti-LTBR antibody [RM2069] - BSA and Azide free (AB320846)

  • 0
  • 0
Proteins & Peptides

AB198752

Recombinant Mouse LTBR protein (Fc Chimera)

SDS-PAGE - Recombinant Mouse LTBR protein (Fc Chimera) (AB198752)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com