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AB218184

Human MMP1 Matched Antibody Pair Kit

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(2 Publications)

Human MMP1 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human MMP1 by sandwich ELISA method.

View Alternative Names

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

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ELISA - Human MMP1 Matched Antibody Pair Kit (AB218184)
  • ELISA

Supplier Data

ELISA - Human MMP1 Matched Antibody Pair Kit (AB218184)

Human MMP1 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Human

Assay type

ELISA set

Sensitivity

= 30.64 pg/mL

Range

125 - 8000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

Human MMP1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MMP1.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab215083. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab218184-5x96Tests", "assetComponentDetails": [ { "size":"1 x 12.5 µg", "name":"Human MMP1 Detector Antibody", "number":"AB218184-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human MMP1 Lyophilized Protein", "number":"AB218184-CMP03", "productcode":"" }, { "size":"1 x 50 µg", "name":"Human MMP1 Capture Antibody", "number":"AB218184-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
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Product protocols

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Target data

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38203564

2024

Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Oliviero,Chiara Baggio,Marta Favero,Amelia Carmela Damasco,Carlotta Boscaro,Davide Tietto,Mattia Albiero,Andrea Doria,Roberta Ramonda

Journal of cosmetic dermatology 20:597-601 PubMed32538515

2020

A noninvasive myorelaxant approach resulting in gummy smile improvement-A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Bojanowski,Pedro Corona,Stephanie Ma,Hui Zhao
View all publications
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websiteProtocolBooklet
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