Human/Monkey C Reactive Protein Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human/Monkey C Reactive Protein.
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Key facts
- Assay type
ELISA set
- Reactive species
Cynomolgus monkey, Rhesus monkey, Human
- Range
18.75 - 1200 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Alternative names
PTX1, CRP, C-reactive protein
What's included?
Recommended products
Human/Monkey C Reactive Protein Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human/Monkey C Reactive Protein.
Key facts
- Assay type
ELISA set
- Reactive species
Cynomolgus monkey, Rhesus monkey, Human
- Range
18.75 - 1200 pg/mL
- Assay Platform
Reagents
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
The Antibody Pair can be used to quantify Human/Monkey C Reactive Protein. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (Human CRP ELISA Kit (C-Reactive protein) ab260058), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
- Biological function summary
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
- Pathways
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
- Associated diseases and disorders
Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human CRP Antibody Pair - BSA and Azide free (ab270347)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human CRP ELISA Kit (C-Reactive protein) ab260058), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
Sandwich ELISA - Human CRP Antibody Pair - BSA and Azide free (ab270347)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human CRP ELISA Kit (C-Reactive protein) ab260058), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
Sandwich ELISA - Human/Monkey C Reactive Protein Antibody Pair - BSA and Azide free (ab270347)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human CRP ELISA Kit (C-Reactive protein) ab260058), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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