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AB314148

Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free

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Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human/Mouse KGF/FGF-7.

View Alternative Names

KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7

2 Images
Sandwich ELISA - Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free (AB314148)
  • sELISA

Supplier Data

Sandwich ELISA - Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free (AB314148)

Sandwich ELISA of ab314148 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Sandwich ELISA - Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free (AB314148)
  • sELISA

Supplier Data

Sandwich ELISA - Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free (AB314148)

Sandwich ELISA of ab314148 with the capture antibody ab314149 dilution at 2 µg/mL and detector antibody ab314150 dilution at 0.5 µg/mL.

Key facts

Reacts with

Human, Mouse

Assay type

ELISA set

Range

1.9 - 250 pg/mL

Assay Platform

Reagents

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human/Mouse KGF/FGF-7 Antibody Pair - BSA and Azide free (ab314148) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human and Mouse KGF/FGF-7 in sandwich ELISAs and many other pair-based applications.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab314148-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human/Mouse KGF/FGF-7 Detector Antibody (unconjugated)", "number":"AB314148-CMP02", "productcode":"AB314150" }, { "size":"1 x 100 µg", "name":"Human/Mouse KGF/FGF-7 Capture Antibody (unconjugated)", "number":"AB314148-CMP01", "productcode":"AB314149" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
See full target information FGF7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com