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AB244189

Human/Mouse NSE Antibody Pair - BSA and Azide free

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Human/Mouse NSE Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human/Mouse NSE.

View Alternative Names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

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Sandwich ELISA - Human/Mouse NSE Antibody Pair - BSA and Azide free (AB244189)
  • sELISA

Supplier Data

Sandwich ELISA - Human/Mouse NSE Antibody Pair - BSA and Azide free (AB244189)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217778) which uses the same antibody pair. For additional information on the performance of pair and kit refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Sandwich ELISA - Human/Mouse NSE Antibody Pair - BSA and Azide free (AB244189)
  • sELISA

Supplier Data

Sandwich ELISA - Human/Mouse NSE Antibody Pair - BSA and Azide free (AB244189)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab217778) which uses the same antibody pair. For additional information on the performance of pair and kit refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

Key facts

Reacts with

Human, Mouse

Assay type

ELISA set

Range

312.5 - 20000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

The Antibody Pair can be used to quantify Human/Mouse NSE. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab217778), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab244189-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human/Mouse NSE Detector Antibody (unconjugated)", "number":"AB244189-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human/Mouse NSE Capture Antibody (unconjugated)", "number":"AB244189-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.
Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.
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Product protocols

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Target data

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
See full target information ENO2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Assay Kits

AB217778

Human Neuron specific Enolase ELISA Kit

elisa-kits

human-neuron-specific-enolase-elisa-kit-ab217778

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