Human Pancreatic Polypeptide antibody Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Pancreatic Polypeptide antibody.
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Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 8 - 500 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pancreatic polypeptide. Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.
Alternative names
PNP, PPY, Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y
What's included?
Recommended products
Human Pancreatic Polypeptide antibody Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Pancreatic Polypeptide antibody.
Key facts
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 8 - 500 pg/mL
- Assay Platform
- Reagents
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PPY Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human PPY in sandwich ELISAs and many other pair-based applications.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pancreatic Polypeptide also known as PP is a small protein with a molecular mass approximately 4200 Da. It is secreted by PP cells in the pancreas particularly in the F-cells located in the Langerhans islets. This peptide is highly conserved among different species including the rat where it displays similar function and expression patterns. Pancreatic polypeptide regulates various physiological processes by binding to specific receptors on target tissues.
- Biological function summary
In various mammalian species including rats pancreatic polypeptide plays a significant role in the regulation of appetite and pancreatic secretion. It is not recognized as part of a multi-protein complex but it directly influences the function of digestive organs by inhibiting pancreatic exocrine secretions and gallbladder contraction. This makes it an important mediator in controlling the body's energy balance and digestive functions.
- Pathways
Pancreatic polypeptide is involved in the peptidergic pathway regulating energy homeostasis and digestive processes. It interacts with the Y receptor family particularly the Y4 receptor which contributes to its regulatory actions on appetite and digestive enzyme secretion. This peptide works alongside other polypeptides such as neuropeptide Y and peptide YY in mediating these complex physiological pathways.
- Associated diseases and disorders
Pancreatic polypeptide levels relate to conditions such as obesity and diabetes. Altered secretion of this peptide can contribute to obesity by affecting appetite regulation and energy homeostasis. Furthermore in diabetes pancreatic polypeptide is connected to abnormal pancreatic function often observed alongside insulin and glucagon dysregulation. Understanding these relationships can provide insight into therapeutic strategies targeting metabolic and endocrine disorders.
Product promise
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1 product image
Sandwich ELISA - Human Pancreatic Polypeptide antibody Antibody Pair - BSA and Azide free (ab253593)
Example of Human Pancreatic Polypeptide standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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