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AB216064

Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit

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(1 Publication)

Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Pro-Collagen I alpha 1 by sandwich ELISA method.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

1 Images
ELISA - Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (AB216064)
  • ELISA

Supplier Data

ELISA - Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (AB216064)

Human Pro-Collagen I alpha 1 standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Human

Assay type

ELISA set

Sensitivity

= 8.44 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Reagents

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Complementary Products

Assay Kits

AB210905

ELISA Accessory Pack

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Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

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AB246841

Human Albumin Matched Antibody Pair Kit

Recombinant

ELISA - Human Albumin Matched Antibody Pair Kit (AB246841)

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Pro-Collagen I alpha 1.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210966. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab216064-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Human Pro-Collagen I alpha 1 Lyophilized Protein", "number":"AB216064-CMP03", "productcode":"" }, { "size":"1 x 12.5 µg", "name":"Human Pro-Collagen I alpha 1 Detector Antibody", "number":"AB216064-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Human Pro-Collagen I alpha 1 Capture Antibody", "number":"AB216064-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen Type 1 also known as Pro-1 collagen is a precursor molecule for collagen Type I which is the most abundant protein in the human body. Its molecular mass is approximately 230 kDa. This protein is synthesized by fibroblasts and is widely expressed in connective tissues including skin tendons and bones. Once synthesized procollagen Type 1 undergoes post-translational modifications and is cleaved by specific enzymes to form mature collagen fibers contributing to the structural integrity of the extracellular matrix.
Biological function summary

The synthesis and processing of procollagen Type 1 play a major role in maintaining tissue strength and elasticity. This molecule is not part of a larger complex but undergoes self-assembly to form the rigid triple-helical structure typical of collagen fibers. These fibers are essential for providing mechanical support to tissues and facilitating cell adhesion. Additionally its presence is often required for normal wound healing processes and tissue regeneration.

Pathways

The secretion and assembly of procollagen Type 1 occur through the secretory pathway. Transforming growth factor-beta (TGF-beta) signaling significantly regulates this process influencing fibrogenesis. This pathway also involves the interaction with proteins such as fibronectin and integrins which are important for aligning collagen fibers within the extracellular matrix. Furthermore procollagen Type 1 engages in the Wnt signaling pathway affecting the arrangement and stability of collagen networks.

Mutations or dysregulation of procollagen Type 1 synthesis may contribute to conditions such as osteogenesis imperfecta and scleroderma. In osteogenesis imperfecta defective collagen leads to brittle bones due to improper fiber formation. In scleroderma an overproduction of collagen causes skin thickening and organ fibrosis. The altered interaction of procollagen Type 1 with proteins like lysyl oxidase can exacerbate these pathological conditions highlighting the significance of proper collagen processing and assembly for tissue health.
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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental orthopaedics 7:48 PubMed32623555

2020

Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome - a diagnostic in vitro tissue remodeling platform.

Applications

Unspecified application

Species

Unspecified reactive species

Marc van Vijven,Bart van Groningen,Joyce N Kimenai,Maria C van der Steen,Marina van Doeselaar,Rob P A Janssen,Keita Ito,Jasper Foolen
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websiteProtocolBooklet
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Product promise

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