Human Procalcitonin Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Procalcitonin by sandwich ELISA method.
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Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 0.98 - 125 pg/mL
- Sensitivity
- = 0.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed:35324283). Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
Alternative names
CALC1, CALCA, Calcitonin
What's included?
Recommended products
Human Procalcitonin Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Procalcitonin by sandwich ELISA method.
Key facts
- Detection method
- Colorimetric
- Assay type
- ELISA set
- Reactive species
- Human
- Range
- 0.98 - 125 pg/mL
- Assay Platform
- Reagents
- Sensitivity
- = 0.2 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Procalcitonin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Procalcitonin.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ELISA Accessory Pack ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit Human Procalcitonin ELISA Kit ab221828. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Procalcitonin also known as PCT is a protein precursor of the hormone calcitonin. It has a molecular mass of about 13 kilodaltons. Procalcitonin is expressed in the thyroid gland typically but can also be produced in other tissues like the liver and kidney during inflammation or sepsis. It gets increasingly produced in response to bacterial infections making it a significant marker for conditions involving systemic inflammation.
- Biological function summary
The function of procalcitonin is related to its precursor status for calcitonin which helps regulate calcium homeostasis. During infections PCT levels rise without its cleavage into active calcitonin indicating that its biological role extends beyond calcium regulation. It does not form part of any larger protein complex in its marker role. When released in response to bacterial infections procalcitonin serves as an indicator for the severity of the infection which is particularly useful in distinguishing bacterial from viral infections.
- Pathways
Procalcitonin gets involved in the immune response pathway primarily due to its expression during bacterial infections. An important pathway is the inflammatory response where procalcitonin production gets triggered serving as a mediator in immune system activation. It is related to cytokines in the immune response which elevated PCT can represent indirectly indicating the presence and level of infection-driven inflammation.
- Associated diseases and disorders
Procalcitonin levels are used significantly in diagnosing and managing sepsis and bacterial infections. Procalcitonin concentrations rise sharply in a bacterial systemic infection and help differentiate it from viral infections an important diagnostic criterion. Additionally in sepsis another important protein involved is C-reactive protein (CRP) often used alongside PCT as a biomarker for inflammatory states in the body. This relationship underlines the importance of PCT in managing infectious diseases through targeted diagnostic pathways.
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1 product image
ELISA - Human Procalcitonin Matched Antibody Pair Kit (ab222276)
Human Procalcitonin standard curve.
Standard calibration curve. Background subtracted values are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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