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AB253842

Human Procollagen III N-terminal propeptide (PIIINP) Antibody Pair - BSA and Azide free

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Human Procollagen III N-terminal propeptide (PIIINP) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Procollagen III N-terminal propeptide (PIIINP).

View Alternative Names

Collagen alpha-1(III) chain, COL3A1

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Sandwich ELISA - Human Procollagen III N-terminal propeptide (PIIINP) Antibody Pair - BSA and Azide free (AB253842)
  • sELISA

Lab

Sandwich ELISA - Human Procollagen III N-terminal propeptide (PIIINP) Antibody Pair - BSA and Azide free (AB253842)

Sandwich ELISA of ab253842 with the capture antibody ab285009 dilution at 2 µg/mL and detector antibody ab285115 dilution at 0.1 µg/mL.

Key facts

Reacts with

Human

Assay type

ELISA set

Range

39.1 - 5000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

Human Collagen III is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human Procollagen III N-terminal propeptide (PIIINP) in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procollagen III N-terminal propeptide known as PIIINP serves as an important marker in collagen synthesis processes. PIIINP results from the cleavage of procollagen III and functions as a byproduct of the maturation of collagen molecules. It possesses a molecular weight of approximately 42 kDa. This target is initially expressed within fibroblast cells found in connective tissues and then secreted into the bloodstream. Given its role in collagen synthesis it is frequently measured to assess the turnover and formation of collagen in tissues.
Biological function summary

This propeptide plays a significant role in the formation of the extracellular matrix. PIIINP is not part of a protein complex but accumulates during the conversion of procollagen into mature collagen fibers which later support tissue structure. Its presence is essential for proper collagen deposition and tissue remodeling. Through its involvement in collagen synthesis PIIINP helps maintain the structural integrity of connective tissues across various organs.

Pathways

Its role reflects the broader functions of collagen metabolism and tissue remodeling pathways. PIIINP marks the processing pathway of procollagen to collagen which is important for the formation of the extracellular matrix. This pathway sometimes intersects with pathways involving other collagens such as collagen I which is another critical component of connective tissues. Together PIIINP and collagen I contribute to the proper maintenance of the extracellular matrix and the mechanical properties of tissues.

Elevated levels of PIIINP often relate to fibrotic conditions and can serve as a biomarker for diseases like liver fibrosis and systemic sclerosis. In liver fibrosis excessive collagen deposition and remodeling occur affecting liver function. In systemic sclerosis abnormal collagen synthesis and deposition in the skin cause thickening and hardening. PIIINP connection to these conditions often involves collagen-binding proteins which regulate collagen stability and deposition.
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Product protocols

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Target data

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1
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