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Human SFRP4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human SFRP4.

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Key facts

Assay type

ELISA set

Reactive species

Human

Range

0.31 - 20 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed:12952927).

Alternative names

What's included?

10 x 96 Tests
Components
Human SFRP4 Capture Antibody (unconjugated)
1 x 100 µg
Human SFRP4 Detector Antibody (unconjugated)
1 x 100 µg

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Human SFRP4 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human SFRP4.

Alternative names

Key facts

Assay type

ELISA set

Reactive species

Human

Range

0.31 - 20 ng/mL

Assay Platform

Reagents

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

  • Human SFRP4 is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human SFRP4 in sandwich ELISAs and many other pair-based applications.
  • The pair can be used in variety of assays and platforms including but not limited to:
  • - Sandwich ELISA
  • - FRET/TR-FRET/HTR
  • - Meso Scale Discovery® (MSD®)
  • - Luminex® and bead-based assays
  • - AlphaLISA®/AlphaScreen®
  • - DELFIA® immunoassays
  • - Simoa® and Single Molecule Counting (SMC™) immunoassays
  • - Multiplex

  • Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
  • - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • - Concentration of ~1 mg/ml as measured by the protein A280 method.

  • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
  • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service

  • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

  • Please note:
  • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
  • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
  • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

  • Antibody properties:
  • Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
  • Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
  • Concentration: ~1 mg/ml
  • Storage buffer: 100% PBS
  • Form: Liquid
  • Isotype: IgG

  • Recombinant monoclonal antibodies offer several advantages including:
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
  • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
  • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
  • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
  • Simoa is a registered trademark of Quanterix, Inc.
  • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Supplementary info

Biological function summary

SFRP4 regulates cell growth and differentiation. It interacts with Wnt ligands preventing them from binding to their receptors. SFRP4 does not typically function as part of a protein complex acting instead as an antagonist on its own. By inhibiting Wnt activity SFRP4 influences embryonic development tissue homeostasis and even cancer progression. The impact of SFRP4 varies depending on the tissue-specific context reflecting its complex biological roles.

Activity summary

SFRP4 also known as Secreted Frizzled Related Protein 4 is a 40 kDa protein involved in modulating Wnt signaling pathways. It is a member of the secreted frizzled-related proteins (sFRPs) that act as Wnt antagonists. SFRP4 has a cysteine-rich domain homologous to the Wnt-binding site on frizzled proteins. Expressed in many tissues including the heart lung and kidney SFRP4 plays a role in various physiological processes by influencing cell signaling.

Pathways

SFRP4 is significant in the Wnt signaling pathway and the β-catenin independent pathway. By interacting with Wnt ligands SFRP4 can prevent the activation of canonical Wnt/β-catenin signaling a pathway involved in cell fate determination and stem cell renewal. It also influences the non-canonical pathways where the Wnt signaling does not involve β-catenin impacting processes like cell movement and polarity. In these pathways SFRP4 interacts with proteins such as Disheveled and Axin modulating their activities.

Associated diseases and disorders

The disruption of SFRP4 expression links to osteoarthritis and breast cancer. Abnormal SFRP4 levels can result in improper Wnt signaling contributing to these conditions. In osteoarthritis SFRP4 imbalance affects cartilage integrity while in breast cancer altered SFRP4 expression may influence tumor growth and progression. Related proteins such as β-catenin in cancer or MMPs in osteoarthritis highlight the importance of SFRP4 in disease susceptibility and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human SFRP4 Antibody Pair - BSA and Azide free (ab253839), expandable thumbnail

    Sandwich ELISA - Human SFRP4 Antibody Pair - BSA and Azide free (ab253839)

    Sandwich ELISA of ab253839 with the capture antibody ab285006 dilution at 2 µg/mL and detector antibody ab285112 dilution at 0.5 µg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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