Human SMOC1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human SMOC1.
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Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
0.16 - 10 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
Alternative names
SPARC-related modular calcium-binding protein 1, Secreted modular calcium-binding protein 1, SMOC-1, SMOC1
What's included?
Recommended products
Human SMOC1 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human SMOC1.
Alternative names
SPARC-related modular calcium-binding protein 1, Secreted modular calcium-binding protein 1, SMOC-1, SMOC1
Key facts
- Assay type
ELISA set
- Reactive species
Human
- Range
0.16 - 10 ng/mL
- Assay Platform
Reagents
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
- Human SMOC1 Antibody Pair - BSA and Azide free (ab309579) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human SMOC1 in sandwich ELISAs and many other pair-based applications.
- The pair can be used in variety of assays and platforms including but not limited to:
- - Sandwich ELISA
- - FRET/TR-FRET/HTR
- - Meso Scale Discovery® (MSD®)
- - Luminex® and bead-based assays
- - AlphaLISA®/AlphaScreen®
- - DELFIA® immunoassays
- - Simoa® and Single Molecule Counting (SMC™) immunoassays
- - Multiplex
- Our antibody pairs are supplied in a a carrier-free format that is conjugation-ready:
- - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- - Concentration of ~1 mg/ml as measured by the protein A280 method.
- Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for biotin, HRP, gold, and fluorescent dyes.
- We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation service
- Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
- Please note:
- The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
- The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
- We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
- Antibody properties:
- Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 μg
- Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 μg
- Concentration: ~1 mg/ml
- Storage buffer: 100% PBS
- Form: Liquid
- Isotype: IgG
- Recombinant monoclonal antibodies offer several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
- Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
- Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
- Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
- AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
- Simoa is a registered trademark of Quanterix, Inc.
- SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
Supplementary info
- Activity summary
SPARC-related modular calcium binding 1 (SMOC1) is an extracellular matrix protein coded by the SMOC1 gene. Known also as its full form the protein weighs approximately 59 kDa and consists of several domains including a thyroglobulin type-1 repeat and two EF-hand domains. SMOC1 is widely expressed in various tissues such as the eye bone and skin. Its expression pattern suggests it plays an important role in tissue development and maintenance.
- Biological function summary
SMOC1 functions involve regulation of angiogenesis and cellular differentiation. It interacts with the extracellular matrix modulating the assembly and dynamics of structural proteins. SMOC1 sounds interactions with calmodulin and integrins forming part of a larger complex. This complex engagement influences cellular processes like cell migration adhesion and stabilization which are pivotal for development and wound healing.
- Pathways
SMOC1 associates with integrin signaling and TGF-beta signaling pathways. These pathways govern processes like cell growth apoptosis and differentiation. SMOC1 modulates the activity of integrins connecting with proteins such as integrin alpha-V beta-3 and transforming growth factor beta (TGF-β). Their relationships indicate SMOC1's involvement in cellular communication and response to environmental changes.
- Associated diseases and disorders
SMOC1 links to conditions like microphthalmia and coloboma as well as bone development disorders. Mutations or dysregulation of SMOC1 impairs normal development indicating its role in ocular and skeletal health. Researchers note connections with proteins like Pax6 which is also involved in eye development. This involvement shows how closely SMOC1 is tied to disorders affecting vision and structural integrity.
Product promise
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1 product image
Sandwich ELISA - Human SMOC1 Antibody Pair - BSA and Azide free (ab309579)
Sandwich ELISA of ab309579 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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