JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB289805

Human Superoxide Dismutase 3/EC-SOD Antibody Pair - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Human Superoxide Dismutase 3/EC-SOD Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Superoxide Dismutase 3/EC-SOD.

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

1 Images
Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD Antibody Pair - BSA and Azide free (AB289805)
  • sELISA

Lab

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD Antibody Pair - BSA and Azide free (AB289805)

Human Superoxide Dismutase 3/EC-SOD Antibody Pair.

Sandwich ELISA of ab289805 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL

Key facts

Reacts with

Human

Assay type

ELISA set

Range

39.1 - 2500 pg/mL

Assay Platform

Reagents

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Superoxide Dismutase 3/EC-SOD Antibody Pair - BSA and Azide free (ab289805) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human Superoxide Dismutase 3/EC-SOD in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab289805-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human Superoxide Dismutase 3/EC-SOD Detector Antibody (unconjugated)", "number":"AB289805-CMP01", "productcode":"AB289821" }, { "size":"1 x 100 µg", "name":"Human Superoxide Dismutase 3/EC-SOD Capture Antibody (unconjugated)", "number":"AB289805-CMP02", "productcode":"AB289820" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.
Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.
style
left-column

Product protocols

style
left-column

Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
See full target information Extracellular superoxide dismutase [Cu-Zn]
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB65354

Superoxide Dismutase Activity Assay Kit (Colorimetric)

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354)

  • 4
  • 2
Assay Kits

AB178012

Human SOD2 ELISA Kit

SimpleStep

Sandwich ELISA - Human SOD2 ELISA Kit (AB178012)

  • 0
  • 0
Assay Kits

AB316899

Human Superoxide Dismutase 3/EC-SOD ELISA Kit

SimpleStep

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

  • 0
  • 0
Primary Antibodies

AB171738

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (AB171738)

  • 0
  • 0
Assay Kits

AB65354

Superoxide Dismutase Activity Assay Kit (Colorimetric)

Functional Studies - Superoxide Dismutase Activity Assay Kit (Colorimetric) (AB65354)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com