Human TIRAP Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human TIRAP.
ELISA set
Human
19.5 - 5000 pg/mL
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6).
MAL, MAL, TIRAP, Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, Adaptor protein Wyatt, MyD88 adapter-like protein, MyD88-2
Human TIRAP Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human TIRAP.
ELISA set
Human
19.5 - 5000 pg/mL
Reagents
Blue Ice
+4°C
+4°C
+4°C
Human TIRAP Antibody Pair - BSA and Azide free (ab309273) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human TIRAP in sandwich ELISAs and many other pair-based applications.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
This supplementary information is collated from multiple sources and compiled automatically.
TIRAP also known as Mal is an adapter protein integral to the Toll-like receptor (TLR) signaling pathway. It has a molecular mass of approximately 24 kDa. TIRAP resides mostly in the cytoplasm and gets recruited to the plasma membrane where it assists in signal transduction. It plays a significant role in bridging TLRs such as TLR2 and TLR4 with downstream signaling elements like MyD88. This protein mediates the recruitment of other signaling proteins after TLR activation therefore facilitating the innate immune response.
TIRAP is part of the innate immune system and contributes to the immune response against pathogens. It forms part of a signaling complex that leads to the activation of NF-kB and MAP kinase pathways resulting in the production of inflammatory cytokines. The protein works by directly interacting with phosphatidylinositol 45-bisphosphate at the plasma membrane through its phosphoinositide-binding motif. This interaction ensures that TIRAP is correctly positioned to relay signals for immune activation.
TIRAP functions in the MyD88-dependent signaling pathway which is significant for initiating inflammatory responses. TIRAP and MyD88 work in tandem to propagate signals from activated TLRs. This relationship not only aids in inflammation but also in the differentiation of immune cells. TIRAP is also related to other proteins like IRAK1 in these pathways which further transduces signals leading to downstream activation of transcription factors relevant for immune responses.
TIRAP plays a part in conditions like systemic lupus erythematosus and septic shock. Mutations in the TIRAP gene can alter the signaling cascade leading to aberrant immune responses associated with these diseases. In this context TIRAP interacts with TLR4 and any dysfunction in the interaction may contribute to the excessive inflammation seen in septic shock. Understanding TIRAP's role in these diseases provides insight into targeted therapeutic strategies.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Sandwich ELISA of ab309273 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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