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AB213467

Human TNF alpha Matched Antibody Pair Kit

5

(1 Review)

|

(2 Publications)

Human TNF alpha Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human TNF alpha by sandwich ELISA method.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
ELISA - Human TNF alpha Matched Antibody Pair Kit (AB213467)
  • ELISA

Unknown

ELISA - Human TNF alpha Matched Antibody Pair Kit (AB213467)

Example of human TNF alpha standard curve in 1X Cell Extraction Buffer PTR using recombinant TNF alpha RabMAb antibody and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Reacts with

Human

Assay type

ELISA set

Sensitivity

= 1.18 pg/mL

Range

1.95 - 1000 pg/mL

Assay Platform

Reagents

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Reactivity data

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Product details

Human TNF alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human TNF alpha.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab181421. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Drug delivery and translational research 13:3030-3058 PubMed37294425

2023

Infliximab microencapsulation: an innovative approach for intra-articular administration of biologics in the management of rheumatoid arthritis-in vitro evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Lamela-Gómez,Lídia M Gonçalves,António J Almeida,Asteria Luzardo-Álvarez

Biosensors & bioelectronics 126:82-87 PubMed30396021

2018

Calorimetric sandwich-type immunosensor for quantification of TNF-α.

Applications

Unspecified application

Species

Unspecified reactive species

Saif Mohammad Ishraq Bari,Louis G Reis,Gergana G Nestorova
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
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Associated Products

Select an associated product type
Alternative Version
Assay Kits

AB241791

Human/Monkey TNF alpha Antibody Pair - BSA and Azide free

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human-monkey-tnf-alpha-antibody-pair-bsa-and-azide-free-ab241791

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(0 reviews)

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